ID   A0A252C059_9PROT        Unreviewed;       489 AA.
AC   A0A252C059;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Radical SAM protein {ECO:0000313|EMBL:OUJ14452.1};
GN   ORFNames=HK28_13405 {ECO:0000313|EMBL:OUJ14452.1};
OS   Acetobacter sp. DsW_063.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Acetobacteraceae; Acetobacter.
OX   NCBI_TaxID=1514894 {ECO:0000313|EMBL:OUJ14452.1, ECO:0000313|Proteomes:UP000195092};
RN   [1] {ECO:0000313|Proteomes:UP000195092}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Winans N.J., Newell P.D., Douglas A.E.;
RL   Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC       sulfatase-maturating enzyme family. {ECO:0000256|ARBA:ARBA00023601}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OUJ14452.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JOPL01000048; OUJ14452.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A252C059; -.
DR   Proteomes; UP000195092; Unassembled WGS sequence.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR023886; QH-AmDH_gsu_maturation.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR   NCBIfam; TIGR03906; quino_hemo_SAM; 1.
DR   NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR   PANTHER; PTHR43273; ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED; 1.
DR   PANTHER; PTHR43273:SF8; RADICAL SAM PROTEIN-RELATED; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR   SFLD; SFLDG01384; thioether_bond_formation_requi; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          107..338
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   489 AA;  53903 MW;  A1A07EE7CA4CA202 CRC64;
     MRHQDALSAS PRYRRGELHE FNAGGNDFIY LVSAGAIFAL DEAARAVLTN MADEGVEHGV
     LVAALCAQGQ QRQDAEDLIR ELVLVQGIVA DASGARQFTS PEAPPTDFPL QALVLNITSQ
     CNLACTYCYE FGSDKIATPQ GKPKFMTLQT AKTSIDFLLE QSAARKAVHV TFFGGETLMN
     FRLLRDVVLY AESQAQQSGK AITFSLTTNA TLLNDEIIDF LSDHHVGVTV SMDGPPDLQN
     KHRVFKNGKG SYDVIEPRLR KLIARHKSRA ITARVTLTDG VTDVVRIFRH LKDDLGFHEV
     GFAPVTSSEK QDYSISDPDM NAVLRGFHDL AEEWLEYALR GQMHGFSNVS ETIGELTQGV
     NKSHPCGAGL GLLGVSPSGD LSPCHRFTDA DSHTLGNINT GIDVEKRGDF LARGHIGTKY
     DCQTCWARPL CAGGCHHEAF VRYGDTGHAN LHYCDWIREW TDTCLKIYGV LAIQKPEFLD
     RFAERKPMT
//