ID A0A254NF42_9BURK Unreviewed; 795 AA.
AC A0A254NF42;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=CDO81_14785 {ECO:0000313|EMBL:OWR03743.1};
OS Roseateles puraquae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Sphaerotilaceae; Roseateles.
OX NCBI_TaxID=431059 {ECO:0000313|EMBL:OWR03743.1, ECO:0000313|Proteomes:UP000197446};
RN [1] {ECO:0000313|EMBL:OWR03743.1, ECO:0000313|Proteomes:UP000197446}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 52769 {ECO:0000313|EMBL:OWR03743.1,
RC ECO:0000313|Proteomes:UP000197446};
RX PubMed=17978231; DOI=10.1099/ijs.0.65149-0;
RA Gomila M., Bowien B., Falsen E., Moore E.R., Lalucat J.;
RT "Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp.
RT nov., isolated from industrial and haemodialysis water.";
RL Int. J. Syst. Evol. Microbiol. 57:2629-2635(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OWR03743.1}.
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DR EMBL; NISI01000005; OWR03743.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A254NF42; -.
DR OrthoDB; 5905204at2; -.
DR Proteomes; UP000197446; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000197446}.
FT DOMAIN 19..300
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 301..601
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 795 AA; 88014 MW; F378B9E4CA7C6095 CRC64;
MNPAPDVDAV AAQHSPLLKN LNPEQLAAVT AAPEPALILA GAGSGKTRVL TTRIAWLLQT
GQVSPGGVMA VTFTNKAAKE MLTRLSTMLP FNVRGMWIGT FHGLCNRFLR AHWKLANLPQ
AFQILDASDT TSAVKRVIKA MNLDEERFVP KQVGWFIAGC KEEALRPGDV EQRDEQTRKL
VEIYANYEDQ CQREGVVDFA ELMLRSYELL RDHPAVREHY QHRFRHILVD EFQDTNKLQY
AWLKMFAPPG AGTGQSLLAV GDDDQSIYAF RGARVGNMAD FEREYRVKRV IKLEQNYRSF
GHILDAANEL ISRNTQRLGK TLRTDAGQGE PIRVNEAPSD FAEAQWLIEE IQQLHRGGLD
RQEIAVLYRS NAQSRVIESA LFNSGIPYRV YGGLRFFERA EVKHALAYLR LLENPNDDTS
FLRVVNFPTR GIGAKTLENL QDAAKASGRS LYQSVSAIAG KGGANLLAFT NLIDSTRNLT
QGMTLREIIE QVLETSGLLD FYRTDKDGAE RLENLDELVN AAEAFVMQEG FGKNAVGLPV
DEVAPPLTAA GAPGAVPDAE TGEIMSPLVA FLTHASLEAG DNQAQAGQDA VQLMTVHSAK
GLEFDAVFIT GLEEGLFPHE NAMSDHDGLE EERRLMYVAI TRARKKLSLS FCQTRMLHGQ
TRYNVKSRFF DELPEESLKW LTPRNQGFGS GFAREYQQAW ERGSGLKGMV GAGRVPQRDQ
RAAWVEPPVP ASMKQKVSEE HGGLRVGKGV FHTKFGEGVL LTLEGAGADA RAQVNFGRHG
TKWLALSVAK LTPID
//
