ID A0A255HYD2_9BURK Unreviewed; 359 AA.
AC A0A255HYD2;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
DE EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
GN Name=rfbB {ECO:0000313|EMBL:OYO31114.1};
GN ORFNames=CD932_08260 {ECO:0000313|EMBL:OYO31114.1};
OS Janthinobacterium sp. PC23-8.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Janthinobacterium.
OX NCBI_TaxID=2012679 {ECO:0000313|EMBL:OYO31114.1, ECO:0000313|Proteomes:UP000216749};
RN [1] {ECO:0000313|EMBL:OYO31114.1, ECO:0000313|Proteomes:UP000216749}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC23-8 {ECO:0000313|EMBL:OYO31114.1,
RC ECO:0000313|Proteomes:UP000216749};
RA Kim H.J., Triplett B.A.;
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC Evidence={ECO:0000256|ARBA:ARBA00001539,
CC ECO:0000256|RuleBase:RU004473};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911,
CC ECO:0000256|RuleBase:RU004473};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily.
CC {ECO:0000256|ARBA:ARBA00008178, ECO:0000256|RuleBase:RU004473}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYO31114.1}.
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DR EMBL; NJGY01000001; OYO31114.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A255HYD2; -.
DR OrthoDB; 9803010at2; -.
DR Proteomes; UP000216749; Unassembled WGS sequence.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR PANTHER; PTHR43000:SF47; DTDP-GLUCOSE 4,6-DEHYDRATASE 2; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU004473}; NAD {ECO:0000256|ARBA:ARBA00023027}.
FT DOMAIN 3..322
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 359 AA; 40203 MW; AF4AEE7D7C346DC6 CRC64;
MILVTGGAGF IGSNFVRDWL AVNDEPVITL DKLTYAGNLS NLASLEQDPR HIFVRGDICD
TSLVSRLLAE HKPRAIVHFA AESHVDRSIH SPGEFISTNV NGTFSLLEAA RAYWSGLTGE
AKEAFRFLHV STDEVYGTLG PQDPPFTETT PYAPNSPYSA SKAASDHLVR AYFHTYGMPV
LTTNCSNNYG SFHFPEKLIP LIISNARAGK PLPIYGDGMQ VRDWLYVGDH CSAIRRVLEA
GRLGEVYNVG GWNEMANLEV VHTLCDILDT LDPKASGSYR EQITYVQDRP GHDRRYAIDA
RKIERELGWK PAETFQTGIR KTVQWYLDNQ AWVRDVQSGD YLKWVEQNYA ARDTTKDAI
//
