ID A0A255Y018_9PROT Unreviewed; 664 AA.
AC A0A255Y018;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 13.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=CHR90_00865 {ECO:0000313|EMBL:OYQ21965.1};
OS Elstera cyanobacteriorum.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Rhodospirillaceae; Elstera.
OX NCBI_TaxID=2022747 {ECO:0000313|EMBL:OYQ21965.1, ECO:0000313|Proteomes:UP000216361};
RN [1] {ECO:0000313|EMBL:OYQ21965.1, ECO:0000313|Proteomes:UP000216361}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TH019 {ECO:0000313|EMBL:OYQ21965.1,
RC ECO:0000313|Proteomes:UP000216361};
RA Cai H.;
RT "Elstera cyanobacteriorum sp. nov., a novel bacterium isolated from
RT cyanobacterial aggregates in a eutrophic lake.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYQ21965.1}.
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DR EMBL; NOXS01000018; OYQ21965.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A255Y018; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000216361; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000216361}.
FT DOMAIN 5..387
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 399..594
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 141
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 313
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 102
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 140
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 321
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 664 AA; 75133 MW; F0626C238F9B9E60 CRC64;
MLGVCYYPEH WNESQWADDA RRMVELGIRI VRIGEFAWTR LEPNPGEYRF EWLDRAIETL
GGAGLKIVLG TPTTTPPKWL IDQMPDMIAY DRDGRPRGFG SRRHYDFSHE GYRREVARIT
EVLAKRYGKN PHIMAWQLDN EYGCHDTIRS YSPVARRAFQ EWLRARYRTI AALNQAWGTV
FWSMELRDFN EAELPNLTVT EANPSAWLDF YRFASDQVVS FNKLKADILR HHAPGQDLSH
NYMGFITEFD HFKVAADLDF ATWDSYPLGF TDTLLRLPPE ERQRWQRTGH PDIAAFHHDL
YRAVGRGRWW VMEQQPGPVN WAFHNPAPLP GMVRLWTWEA FAHGAEVVTY FRWRQVPFAQ
EQMHAGLLRP DSEPAPGYAE AGAVAVEAAD LPMPDTEPAP VAILFDYAAD WLLTAHPQGR
EYSFKHIVFD WYQAVRRLGL DVDFVSPDGD PSAYRLILAP ASPLVSDALA ARLAQAVASG
ATLVLGPRVG SKTENLHIPS NLAPGALATL AGFRVERVES LDPAFAEPVT WHGETYQTRG
WREATEGGAK VLATFNDGWP ALREQRHGAG RVYSWHGLPD GDFLRAVLAP WAEAVGLTLH
PVPETVRLRR RGSLTFAFNY GSDPWHGPVE GASGVRYRIG GARVLPQQIA AWERSPSHDP
MGVE
//
