ID A0A255Z677_9PROT Unreviewed; 507 AA.
AC A0A255Z677;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=CHU95_02370 {ECO:0000313|EMBL:OYQ37043.1};
OS Niveispirillum lacus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Niveispirillum.
OX NCBI_TaxID=1981099 {ECO:0000313|EMBL:OYQ37043.1, ECO:0000313|Proteomes:UP000216998};
RN [1] {ECO:0000313|EMBL:OYQ37043.1, ECO:0000313|Proteomes:UP000216998}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TH1-14 {ECO:0000313|Proteomes:UP000216998};
RA Cai H.;
RT "Niveispirillum cyanobacteriorum sp. nov., isolated from cyanobacterial
RT aggregates in a eutrophic lake.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYQ37043.1}.
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DR EMBL; NOXU01000018; OYQ37043.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A255Z677; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000216998; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR045443; DUF6504.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF20114; DUF6504; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000216998};
KW Transferase {ECO:0000313|EMBL:OYQ37043.1}.
FT DOMAIN 29..150
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 246..328
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
FT DOMAIN 431..504
FT /note="DUF6504"
FT /evidence="ECO:0000259|Pfam:PF20114"
SQ SEQUENCE 507 AA; 55448 MW; 0593255B75A4F97C CRC64;
MNRVVSLYLP NWPIDRLRRA AGDAAPPADR PLVLAGRIRN RRVVTACCQA ALEAGLRPGM
ALAKAQILVP ELVVNEAAPQ ADQEGLYRLA LWILQRFSPI VAADPPDGIV IDTTGADHLH
GGEEVMLEAL VGRLAMSGVE ARAAVADSWG AAHAFARYLA KPTFVADPGT GLSLLSPLPL
EALRLPVAMA GDLRTLGFRT VGELHRQPRA PLALRFGPQL ARRIDQALGE AAEPFDPVRP
ADQVDARRAF AEPIAAAETI ARYIGKLVVD LCAALEERGL GARRLDLLVH RVDSRLQAVR
VGMALPTRDV RRLTRLLCDK IETIDPGFGI EAMVLVAAIA EPLEMKQAVN SLMEAARPDV
SGLVDTLANR IGGRAVYRIA AVASDVPERS VQRIPAMAPD TGTGWPDHWP RPARLLARPE
PIETVALLPD HPPASFTWRG VRRRVRRADG PERVFGEWWK RDAELEAVRD YFRVEDDAGE
RYWVYRAGDG EDPATGSHRW FLHGIFG
//
