ID A0A255ZDB8_9FLAO Unreviewed; 524 AA.
AC A0A255ZDB8;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Signal peptidase I {ECO:0000256|ARBA:ARBA00013208, ECO:0000256|RuleBase:RU362042};
DE EC=3.4.21.89 {ECO:0000256|ARBA:ARBA00013208, ECO:0000256|RuleBase:RU362042};
GN ORFNames=CHX27_14470 {ECO:0000313|EMBL:OYQ38590.1};
OS Flavobacterium aurantiibacter.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2023067 {ECO:0000313|EMBL:OYQ38590.1, ECO:0000313|Proteomes:UP000216035};
RN [1] {ECO:0000313|EMBL:OYQ38590.1, ECO:0000313|Proteomes:UP000216035}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TH167 {ECO:0000313|EMBL:OYQ38590.1,
RC ECO:0000313|Proteomes:UP000216035};
RA Cai H.;
RT "Flavobacterium cyanobacteriorum sp. nov., isolated from cyanobacterial
RT aggregates in a eutrophic lake.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences
CC from secreted and periplasmic proteins.; EC=3.4.21.89;
CC Evidence={ECO:0000256|ARBA:ARBA00000677,
CC ECO:0000256|RuleBase:RU362042};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|RuleBase:RU362042}; Single-
CC pass type II membrane protein {ECO:0000256|RuleBase:RU362042}.
CC -!- SIMILARITY: Belongs to the peptidase S26 family.
CC {ECO:0000256|ARBA:ARBA00009370, ECO:0000256|RuleBase:RU362042}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU362042}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYQ38590.1}.
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DR EMBL; NOXX01000226; OYQ38590.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A255ZDB8; -.
DR OrthoDB; 9802919at2; -.
DR Proteomes; UP000216035; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR CDD; cd06530; S26_SPase_I; 2.
DR Gene3D; 2.10.109.10; Umud Fragment, subunit A; 2.
DR InterPro; IPR043739; DUF5684.
DR InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR InterPro; IPR000223; Pept_S26A_signal_pept_1.
DR InterPro; IPR019758; Pept_S26A_signal_pept_1_CS.
DR InterPro; IPR019533; Peptidase_S26.
DR NCBIfam; TIGR02227; sigpep_I_bact; 2.
DR PANTHER; PTHR43390:SF1; CHLOROPLAST PROCESSING PEPTIDASE; 1.
DR PANTHER; PTHR43390; SIGNAL PEPTIDASE I; 1.
DR Pfam; PF18936; DUF5684; 1.
DR Pfam; PF10502; Peptidase_S26; 2.
DR PRINTS; PR00727; LEADERPTASE.
DR SUPFAM; SSF51306; LexA/Signal peptidase; 1.
DR PROSITE; PS00761; SPASE_I_3; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU362042};
KW Membrane {ECO:0000256|RuleBase:RU362042};
KW Protease {ECO:0000256|RuleBase:RU362042};
KW Reference proteome {ECO:0000313|Proteomes:UP000216035};
KW Transmembrane {ECO:0000256|RuleBase:RU362042};
KW Transmembrane helix {ECO:0000256|RuleBase:RU362042}.
FT TRANSMEM 6..25
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362042"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362042"
FT TRANSMEM 89..111
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362042"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362042"
FT TRANSMEM 500..519
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362042"
FT DOMAIN 125..291
FT /note="Peptidase S26"
FT /evidence="ECO:0000259|Pfam:PF10502"
FT DOMAIN 429..469
FT /note="Peptidase S26"
FT /evidence="ECO:0000259|Pfam:PF10502"
FT ACT_SITE 153
FT /evidence="ECO:0000256|PIRSR:PIRSR600223-1"
FT ACT_SITE 262
FT /evidence="ECO:0000256|PIRSR:PIRSR600223-1"
SQ SEQUENCE 524 AA; 59938 MW; 45A0F9E022FD1C2D CRC64;
MTFSEWLIVI LIFQIIHFLG TWKLYVRAGR KAWEAAIPIY NAIVLMKIIN RPTYWVILLF
VPVVNLIMFP VIWVETARSF GKNTLTDTWL TLLTFGFYLY VLNYAANLTY VENRSLIART
KTGDTVSSIL FAIVVATLVH TYVMQPFQIP TSSLEKTLLV GDFLFVSKFH YGARPQMSAV
SFPMVHDTIP VAKVKSYVYD DANKDSWKNK LQYPYVRIPG FQTIKNNDIV VFNWPCDTLF
HMYERADRRY DKPIDKKTNY VKRCVGIAGD KLEIKQGTVY INGKAVVYPE RAKIQYVYQA
AFDRSKPVDF NYVMKTIGST EQVGSNTKGD TIFFSALTEE GVEKLKSFSA FSGIKRRIET
RTDSRIFGGQ LSWNADNMGP IYIPKAGTTI ALTPENLPFY KKVITEYEGP HLVKVVGNDV
YIDNKLAKSY TFQKDYYWMM GDNRGNSLDS RYWGFVPEDH VVGKAVFVWL SLDGTASGLD
KIRWSRMFTT VNGDGPATSY LWVFGLAVLA YFGLTRFVFK KKAA
//
