ID   A0A256CTD6_9GAMM        Unreviewed;       365 AA.
AC   A0A256CTD6;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   24-JAN-2024, entry version 21.
DE   SubName: Full=Hydrolase {ECO:0000313|EMBL:OYQ81357.1};
GN   ORFNames=B9T19_01400 {ECO:0000313|EMBL:OYQ81357.1};
OS   Ignatzschineria sp. F8392.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Cardiobacteriales;
OC   Ignatzschineriaceae; Ignatzschineria.
OX   NCBI_TaxID=1980117 {ECO:0000313|EMBL:OYQ81357.1, ECO:0000313|Proteomes:UP000215803};
RN   [1] {ECO:0000313|EMBL:OYQ81357.1, ECO:0000313|Proteomes:UP000215803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=F8392 {ECO:0000313|EMBL:OYQ81357.1,
RC   ECO:0000313|Proteomes:UP000215803};
RA   Gulvik C.A.;
RT   "Wohlfahrtiimonas and Ignatzschineria genomes.";
RL   Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OYQ81357.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; NEFF01000001; OYQ81357.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A256CTD6; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000215803; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF0; AMINOPEPTIDASE YPDE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:OYQ81357.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670}.
FT   ACT_SITE        213
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         69
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         181
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         181
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         214
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         236
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         332
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   365 AA;  39357 MW;  196ABEEC95E9CB1C CRC64;
     MANLKENIKS LLKDLTHINA GVGQEQPVIS YIMSALKGEV DELKVDHNGN VHAIKRGHKE
     GLTMMVAAHT DEIGLIVKNI LPNGFLLIEK LGGVPDNMLL GRKVYVGKNN LPGVIGTKPG
     HLQTAEEAKR VKTVAECYVD MALPSREAVE AHGVSVGDQV IISGEFTEMV DPDYISMKAI
     DDRLGCSILI ELLKNLSKQD FAGTLHGVFT VQEEVGLYGA QKVGDGYTPD YAIVLDTIPA
     GDTPDVNTER DLPVRLGAGP ALPLADAVMP IFFSMVHPAV RRAIEKQATT QNIHLQKLTL
     LGGGYTTDSA KLSHAGGGIP CATLAIPRRY SHSPIELTNL NDSVDVYNIL QGLIRDNENM
     NTAFI
//