ID A0A257C6Z5_9BURK Unreviewed; 679 AA.
AC A0A257C6Z5;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE SubName: Full=Alkaline phosphatase {ECO:0000313|EMBL:OYT84967.1};
GN ORFNames=CFE46_18795 {ECO:0000313|EMBL:OYT84967.1};
OS Burkholderiales bacterium PBB6.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
OX NCBI_TaxID=2015568 {ECO:0000313|EMBL:OYT84967.1, ECO:0000313|Proteomes:UP000216978};
RN [1] {ECO:0000313|EMBL:OYT84967.1, ECO:0000313|Proteomes:UP000216978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PBB6 {ECO:0000313|EMBL:OYT84967.1};
RA Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT formosa and its bacterial cohort.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 1 Mg(2+) ion. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- SIMILARITY: Belongs to the alkaline phosphatase family.
CC {ECO:0000256|RuleBase:RU003946}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYT84967.1}.
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DR EMBL; NKIH01000008; OYT84967.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A257C6Z5; -.
DR Proteomes; UP000216978; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IEA:InterPro.
DR CDD; cd16012; ALP; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 2.
DR InterPro; IPR001952; Alkaline_phosphatase.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR PANTHER; PTHR11596; ALKALINE PHOSPHATASE; 1.
DR PANTHER; PTHR11596:SF72; ALKALINE PHOSPHATASE; 1.
DR Pfam; PF00245; Alk_phosphatase; 1.
DR PRINTS; PR00113; ALKPHPHTASE.
DR SMART; SM00098; alkPPc; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
PE 3: Inferred from homology;
KW Magnesium {ECO:0000256|PIRSR:PIRSR601952-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601952-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000216978};
KW Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR601952-2}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..679
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012287724"
FT ACT_SITE 213
FT /note="Phosphoserine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-1"
FT BINDING 164
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 164
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 272
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 274
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 453
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 458
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 462
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 500
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 501
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 628
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
SQ SEQUENCE 679 AA; 72831 MW; 0DFE09C5CC7FD019 CRC64;
MSHPRLSRTL FMAPLGCLFI AATAAQAAPV VSRLTPPSEL FSSGQPEPVI ARFLPGQRFD
LQATLRPDGG QRITEARFEV DGKRVAGDVA LRDCETGCLK GVPQNAAIAT LRAVTVTAPG
RHEFRVLARQ ADGQSVVARG NFDVVPFQTP SGQKVRNIII LLGDGMGASH RTAARIVQGR
YAQGKVSSPL AMDTFPATAL VKTSSLNSVV TDSSPGMTAY VLGNKNNNNE EGVFPDDTVD
AFDNPRIEYL SEYLHRLQGK ALGIVTTADV FDSTPAGNAV HTSSRGAGTG IVDQFLDDRH
ATGLQVLMGG GRRWFLPAGT VGSSRDERTD YAFSSTEPHT AEIVRRWGVS AGAVDTGRDL
IADFQQAGFR YAANRQAMDT LPVDTDKVLG LFAFSNMNVA LDKIDGRRGR RTGVSGTVVD
DFGLPDQPML DEMATVALRA LSRQPKGFVL MVEGASIDKQ AHNMDTERWM LDTLEFDRAV
KVAQDYARQH ADTLVIVTAD HECSGAAVIG GSRVSDQALQ ELIKAGGPEN LRDKVVGVYE
QAGFPRYQLA ADGYPETTNI DRRVLVGYAA NADRYEDWRT NPLPLRSGGA PFVQVEPLKL
YPAGPLQRDD AEGRFLITGQ VPGDSAVHTA TDVPLSAYGP GALTFTGVID NTDVFFKLAQ
AAMRGTVAPA DASGSKKPR
//
