ID A0A257FIY2_9BURK Unreviewed; 499 AA.
AC A0A257FIY2;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE SubName: Full=Deoxyribodipyrimidine photolyase {ECO:0000313|EMBL:OYU26093.1};
GN ORFNames=CFE41_17885 {ECO:0000313|EMBL:OYU26093.1};
OS Burkholderiales bacterium PBB2.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
OX NCBI_TaxID=2015564 {ECO:0000313|EMBL:OYU26093.1, ECO:0000313|Proteomes:UP000215624};
RN [1] {ECO:0000313|EMBL:OYU26093.1, ECO:0000313|Proteomes:UP000215624}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PBB2 {ECO:0000313|EMBL:OYU26093.1};
RA Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT formosa and its bacterial cohort.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate;
CC Xref=ChEBI:CHEBI:15636; Evidence={ECO:0000256|ARBA:ARBA00001932};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|PIRSR:PIRSR602081-1};
CC Note=Binds 1 FAD per subunit. {ECO:0000256|PIRSR:PIRSR602081-1};
CC -!- SIMILARITY: Belongs to the DNA photolyase family.
CC {ECO:0000256|RuleBase:RU004182}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYU26093.1}.
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DR EMBL; NKIM01000032; OYU26093.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A257FIY2; -.
DR Proteomes; UP000215624; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.80; -; 1.
DR Gene3D; 1.10.579.10; DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR036134; Crypto/Photolyase_FAD-like_sf.
DR InterPro; IPR036155; Crypto/Photolyase_N_sf.
DR InterPro; IPR005101; Cryptochr/Photolyase_FAD-bd.
DR InterPro; IPR002081; Cryptochrome/DNA_photolyase_1.
DR InterPro; IPR006050; DNA_photolyase_N.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR11455; CRYPTOCHROME; 1.
DR PANTHER; PTHR11455:SF9; CRYPTOCHROME-1; 1.
DR Pfam; PF00875; DNA_photolyase; 1.
DR Pfam; PF03441; FAD_binding_7; 1.
DR PRINTS; PR00147; DNAPHOTLYASE.
DR SUPFAM; SSF48173; Cryptochrome/photolyase FAD-binding domain; 1.
DR SUPFAM; SSF52425; Cryptochrome/photolyase, N-terminal domain; 1.
DR PROSITE; PS51645; PHR_CRY_ALPHA_BETA; 1.
PE 3: Inferred from homology;
KW Chromophore {ECO:0000256|RuleBase:RU004182};
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR602081-1};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630, ECO:0000256|PIRSR:PIRSR602081-
KW 1}; Lyase {ECO:0000313|EMBL:OYU26093.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000215624}.
FT DOMAIN 19..150
FT /note="Photolyase/cryptochrome alpha/beta"
FT /evidence="ECO:0000259|PROSITE:PS51645"
FT BINDING 242
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-1"
FT BINDING 289
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-1"
FT BINDING 292..299
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-1"
FT BINDING 392..394
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-1"
FT SITE 323
FT /note="Electron transfer via tryptophanyl radical"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-2"
FT SITE 379
FT /note="Electron transfer via tryptophanyl radical"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-2"
FT SITE 402
FT /note="Electron transfer via tryptophanyl radical"
FT /evidence="ECO:0000256|PIRSR:PIRSR602081-2"
SQ SEQUENCE 499 AA; 56075 MW; BEA31A88AD23D8CC CRC64;
MTRPINASDP SPAPSSRVPR ALVWFRRDLR VDDQAALYRA LRAAQQVFCV FVLDRDILDA
LPRSDRRVDF ILRSLVELDA GLRALCPGGG LIVRHGRAAE DIPRLAAELE VQAVFCNHDD
EPAALARDAQ VAEALASFAC QLRSFKDHVV FERNEVLTGA GKPYGVFTPY KNAWLRQINE
FYLSSYPVAR HAAGLAPTAL ARGVPSLAEI GFEPTDLSPH LGAGASGAEA LFEEFLDRMD
QYEASRNFPA VKGPSYLSLH LRFGTISIRR LAREAWQRAQ GGDRGAEVWL SELIWRDFYH
QVMHHHPHAM VRAFRPEYDA ITWDSGPAAD HLFKAWCEGR TGYPLVDAAM LQLNQSGYMH
NRLRMVTACF LIKDLGIDWR RGEAYFAEKL LDFDLAANNG GWQWASSSGC DAQPYFRIFN
PVTQSEKFDP EGKFIKRYLP QLAKLPPKLI HAPWLAKPLE LEACRLVLGR DYPTPVVRHD
EARAATLARY AVVKSRAIE
//
