ID A0A257FSZ7_9BURK Unreviewed; 934 AA.
AC A0A257FSZ7;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=CFE41_03735 {ECO:0000313|EMBL:OYU28729.1};
OS Burkholderiales bacterium PBB2.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
OX NCBI_TaxID=2015564 {ECO:0000313|EMBL:OYU28729.1, ECO:0000313|Proteomes:UP000215624};
RN [1] {ECO:0000313|EMBL:OYU28729.1, ECO:0000313|Proteomes:UP000215624}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PBB2 {ECO:0000313|EMBL:OYU28729.1};
RA Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT formosa and its bacterial cohort.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYU28729.1}.
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DR EMBL; NKIM01000004; OYU28729.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A257FSZ7; -.
DR Proteomes; UP000215624; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR024561; Pullul_strch_C.
DR InterPro; IPR040671; Pullulanase_N2.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF11852; Pullul_strch_C; 1.
DR Pfam; PF17967; Pullulanase_N2; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 2.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000215624};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..934
FT /note="pullulanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012626615"
FT DOMAIN 412..770
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 934 AA; 101074 MW; E80ED810FC3C88B5 CRC64;
MTSLSSSRLG LILALNLLSS ATWAGSVTAA SAAELRAACD SADAATVLQP AAMAAPEARA
FALSPALLQW PGQSLGPGER VALLASARGG VQVKVGAAAQ GVEQRVELTP SEAPLPPELA
QRFKYLESFG PGLRLALTVA DQARLPQLLR GELLLVKEDA AGQVLAVTGL QTSGALDALY
ADAAEAAGEL GAVVVKKGRQ TEFKLWAPTA QQVYLCRYAG PESPAQDVDA LALDARSGLW
QQRLPANLSG SYYRYLVEVW VPGLGRVRNR VTDPYSLSLS ADSARSMVVD WADPRLQPPG
WQDRRAHRQA SARVRQSTDM VIYELHVRDF SIGDATVPPA QRGKYLAFTQ AGSQGMRHLR
ALAQVGLTDV HLLPVFDLAS VPEQGCLTPQ VPEAAADSPA QQAAVMKVAG QDCFNWGYDP
WHYSAPEGSY ASDAQRGERR IREFRKMVQA LHRAGLRVGM DVVYNHTAAS GQGRHSVLDR
IVPGYYQRLD AKGHVERSTC CDNTATEQRM MAKLMIDSVA MWAREYRIDS FRFDLMAHQP
RAVMEALQQR VNAAAGQPVQ LIGEGWNFGE VANGARFVQA SQLSLNGSGI GTFSDRARDA
ARGGSAGDNG LDVLKNQGWL NGLGFAPNRH VGTAAAATQL MASADLLRVG LAGSLRDFEL
DTWRGPRLRA SEIAYGDQPA GYVSEPGEVV NYVDNHDNQT LFDINVLKLP QDTPRAERAR
VQLLGAALTA FSQGVAYFHA GIDVLRSKSL DRNSYDSGDW FNRLDWSYQR NFFGTGLPPA
ADNQAMYPWL APLLADPGIA PGPAEIAWMR DGFRDLLKIR ASSRLLRLSS AAEIRQRLRF
LNTGPAQNPM VLAAEIDGQG LKDAGFARLA YFLNTSPEAQ VLQLPELAGR RYALHPVHLA
PGAADPRPKR EFAFEAATGQ LKVPGRTALV LVER
//
