ID   A0A257HYE9_9BACT        Unreviewed;       822 AA.
AC   A0A257HYE9;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Penicillin acylase family protein {ECO:0000313|EMBL:OYU55637.1};
GN   ORFNames=CFE25_09890 {ECO:0000313|EMBL:OYU55637.1};
OS   Chitinophagaceae bacterium BSSC1.
OC   Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae.
OX   NCBI_TaxID=2015574 {ECO:0000313|EMBL:OYU55637.1, ECO:0000313|Proteomes:UP000217121};
RN   [1] {ECO:0000313|EMBL:OYU55637.1, ECO:0000313|Proteomes:UP000217121}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BSSC1 {ECO:0000313|EMBL:OYU55637.1};
RA   Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA   Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT   "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT   formosa and its bacterial cohort.";
RL   Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OYU55637.1}.
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DR   EMBL; NKJC01000002; OYU55637.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A257HYE9; -.
DR   Proteomes; UP000217121; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000217121};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        288
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         360
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         363
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   822 AA;  93134 MW;  E97E5BD5151E91E6 CRC64;
     MRLLPFIAST AITIGLVTVL NMQIPLGASK TPRLGYFLSP QQGFWQNAEP TNSSFNAQIN
     TRGLKNKVDV YMDERLVPHI YAQNDLDAYY VQGFLHAKFR LWQMEFQTYV ASGRLCEILG
     DSKLDIDRYF RRMGMVYGAE QTMAKMDNNR EMQATVDAYT EGVNAYIKLL KPEDLPFEYK
     LLDYRPENWT PLKTYLFLMF MSYDLSGRGV SSDLKMTNAR DYLGFESFNQ LFPNLQDSLD
     PILPPSTIYG KPSIKPVKPA KSDSLYLHQV KATVNVSSPA PPNKNNGSNN WAVSGKKTRS
     GRPILCNDPH LGLNLPSLWY EVQINTPTHN TYGASFPGSP AVIIGFNDSI AWGVTNAGRD
     VFDYYDMHFR DSTMKEYWYN GVWKKAEQRV EVIKIKGKPE IEEKIAMTVY GPVMYDHSYS
     NESTKGRSLA VAWTANIAGN GLQTFYKLNR AKNFDDYQSA ISNWWCPGQN FAFASKSGDI
     AIKQQGAFVA RWNRQGDFIM PGSDSSFNWQ GIIPENENPM VRNPARQFVS SANQMSTDTS
     YPYYLGGASA FPIYRGIIIN RKLAVLNDIT TTDMQMLQME NVNVFAEMAR PVLLKYINES
     KLTKPQKKYV EILKNWNLRN DITEQGITVF NNVWDSLENV VWKDDFQKAA KPMPWPESST
     LLEHLLKDSV YAYADNILTT TKTETIQDAV MEAVVRASAA MDKMVMEGKK LDWGAYKDTK
     VEHLLKISAL SSLHLPIGGG ENCINATGTV HGPSWRMIVQ MTDVIEAYGV YPGGQNGNPG
     SKFYDNFINS WAAGKYYRLL FISKEDVTKQ EKIKWHIAFI NA
//