ID A0A257KFY1_9FLAO Unreviewed; 755 AA.
AC A0A257KFY1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Citrate hydro-lyase {ECO:0000256|ARBA:ARBA00029682};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=CFE24_01775 {ECO:0000313|EMBL:OYU85440.1};
OS Flavobacterium sp. BFFFF2.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2015558 {ECO:0000313|EMBL:OYU85440.1, ECO:0000313|Proteomes:UP000216176};
RN [1] {ECO:0000313|EMBL:OYU85440.1, ECO:0000313|Proteomes:UP000216176}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BFFFF2 {ECO:0000313|EMBL:OYU85440.1};
RA Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT formosa and its bacterial cohort.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYU85440.1}.
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DR EMBL; NKJD01000003; OYU85440.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A257KFY1; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000216176; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015932; Aconitase_dom2.
DR InterPro; IPR006248; Aconitase_mito-like.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01340; aconitase_mito; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000216176}.
FT DOMAIN 34..477
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 558..686
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 755 AA; 80877 MW; 141269ADA9638C2E CRC64;
MAFDIEMIKK AYAQMPARVD QARAVVGRPL TLSEKILYAH LWENNPSHAY ARGKDYVDFA
PDRVACQDAT AQMALLQFMH AGKNKVAVPT TVHCDHLIQA KVGAKTDLEV ASKQSKEVFD
FLSSVSNKYG IGFWKPGSGI IHQIVLENYA FPGGMMIGTD SHTVNAGGLG MLAIGVGGAD
AVDVMSGMSW ELKFPKLIGV KLTGKLSGWT APKDVILKVA DILTVKGGTG AIVEYFGEGA
EAMSCTGKGT ICNMGAEIGA TTSTFGYDDS MRRYLTATGR ADVVAAADEV AAYLTADPEV
YADPATYFDQ LIEINLSELE PHINGPFTPD RGTPVSKMKQ EAEANGWPLA VEWGLIGSCT
NSSYEDMARA ASIVEQAVAH GITPKAEFGI NPGSEQIRFT IERDGIIATF EKMGTKVFTN
ACGPCIGQWD RTGADKGEKN TIVHSFNRNF SKRADGNPNT HAFVTSPEMV AALAISGRLD
FNPITDTLLN DKGEAVRLLP PTGDELPTRG FDVDDAGFQA PAADGSGIQV AVSVTSDRLQ
LLEPFQAWDG HNISGAKLLI KAFGKCTTDH ISMAGPWLRY RGHLDNISNN MLIGAVNAYT
QQSNSVKNQL TGGYDAVPAV QRAYKAANVP SIVVGDHNYG EGSSREHAAM EPRFLGVKAV
LVKSFARIHE TNLKKQGMLA LTFANEADYD KIQEDDTFNF TDLTSFAPGK PLTLEVVHAD
GSADTILANH TYNDSQIGWF KAGSALNLIA AAGAA
//
