ID A0A257KJC8_9BRAD Unreviewed; 755 AA.
AC A0A257KJC8;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN Name=ptsP {ECO:0000313|EMBL:OYU87349.1};
GN ORFNames=CFE29_25470 {ECO:0000313|EMBL:OYU87349.1};
OS Bradyrhizobiaceae bacterium PARB1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae.
OX NCBI_TaxID=2015576 {ECO:0000313|EMBL:OYU87349.1, ECO:0000313|Proteomes:UP000216768};
RN [1] {ECO:0000313|EMBL:OYU87349.1, ECO:0000313|Proteomes:UP000216768}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PARB1 {ECO:0000313|EMBL:OYU87349.1};
RA Kojadinovic M., Villain A., Puppo C., Fon Sing S., Prioretti L., Hubert P.,
RA Gregori G., Zhang Y., Sassi J.-F., Claverie J.-M., Blanc G., Gontero B.;
RT "A metagenomic investigation of the 'star' freshwater diatom Asterionella
RT formosa and its bacterial cohort.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYU87349.1}.
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DR EMBL; NKIY01000009; OYU87349.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A257KJC8; -.
DR Proteomes; UP000216768; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR PANTHER; PTHR46244:SF3; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:OYU87349.1};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OYU87349.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 24..170
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
SQ SEQUENCE 755 AA; 82437 MW; B719B670920AE7A8 CRC64;
MRSASGGPRV LLRRLREVMA EQVSAQERLD KIVVLIAANM VAEVCSTYVL RVDNTLELYA
TEGLNREAVH RTVLNAHEGL VGLVASEATP LNLNDAQSHP AFAFRPETGE EIYHSFLGVP
ILRAGNTLGV LVVQNRAKRT YVEEEVEALQ TTAMVLAEMI ASGELSSLAQ PGAEPAARHS
IHKTGAILSD GIALGHVVLH EPRVVITNYI AEDLPKEVKR LDTALTKLRA DLDRMLERGD
VAESGEHRDV LEAYRMFAND HGWSHKLHEA VATGLTAEAA VERVQSDTRA RMLRSTDPYL
RDRLHDLEDL GHRLMRQLVG QDHAPSREQM PDNAILIARA MGPAALLDYD RKKLRGLVLE
EGTANSHVSI VARALGIPAL GEVANAPGIA DPGDAIIVDG TSGSIYIRPS QEIESAYAER
VRFRARRQAQ YSALRDKPCV TKDGVKVDLL INAGLIIDLP HIDDTGSAGI GLFRTELQFM
VGESLPRSSD QLQLYRAVLD AAGPKPVTFR TLDIGGDKAL PYMETVVEEN PALGWRAIRL
GLDRPGLLRG QIRALLRAGG GRALRIMFPM ISEVAEFDQA KAIIERELTY LRQHGHALPE
RIDVGTMIEV PALVYQLDEL LKKVDFVSVG SNDLFQFLFA VDRGNSKVSD RFDTLSAPIL
RVLREIVQKG DTAKRHVSLC GEMASQPIGA LALVALGYRT LSLSATAHGP VKALILDLDA
KKAQAAMAKW LDAPSGSISI RQKLTEFAEA EGLSL
//
