ID A0A258CM32_9GAMM Unreviewed; 72 AA.
AC A0A258CM32;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase E {ECO:0000256|ARBA:ARBA00041129};
DE EC=2.1.1.166 {ECO:0000256|ARBA:ARBA00038861};
DE AltName: Full=23S rRNA Um2552 methyltransferase {ECO:0000256|ARBA:ARBA00042745};
DE AltName: Full=rRNA (uridine-2'-O-)-methyltransferase {ECO:0000256|ARBA:ARBA00041995};
DE Flags: Fragment;
GN ORFNames=B7Z23_00290 {ECO:0000313|EMBL:OYW96626.1};
OS Pseudomonadales bacterium 32-61-5.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales.
OX NCBI_TaxID=1970544 {ECO:0000313|EMBL:OYW96626.1, ECO:0000313|Proteomes:UP000215582};
RN [1] {ECO:0000313|EMBL:OYW96626.1, ECO:0000313|Proteomes:UP000215582}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=32-61-5 {ECO:0000313|EMBL:OYW96626.1};
RA Kantor R.S., Colenbrander Nelson T., Marshall S., Bennett D., Apte S.,
RA Camacho D., Thomas B.C., Warren L.A., Banfield J.F.;
RT "Lifting the veil on microbial sulfur biogeochemistry in mining
RT wastewaters.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the uridine in position 2552 of 23S
CC rRNA at the 2'-O position of the ribose in the fully assembled 50S
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00037569}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-
CC methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42720, Rhea:RHEA-COMP:10202, Rhea:RHEA-COMP:10203,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.166;
CC Evidence={ECO:0000256|ARBA:ARBA00036915};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYW96626.1}.
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DR EMBL; NCDF01000010; OYW96626.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A258CM32; -.
DR Proteomes; UP000215582; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR10920; RIBOSOMAL RNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10920:SF18; RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL; 1.
DR Pfam; PF01728; FtsJ; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:OYW96626.1};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OYW96626.1}.
FT DOMAIN 29..70
FT /note="Ribosomal RNA methyltransferase FtsJ"
FT /evidence="ECO:0000259|Pfam:PF01728"
FT NON_TER 72
FT /evidence="ECO:0000313|EMBL:OYW96626.1"
SQ SEQUENCE 72 AA; 8184 MW; 0F4DA3CD9210BCBC CRC64;
MKRSKSSRRW LDEHVNDPYV KQAQKDGLRS RSSYKLIELN EKDKLIRPGM LLMDLGSAPG
GWSQVAGRLV GE
//
