ID A0A258TTN5_9PROT Unreviewed; 283 AA.
AC A0A258TTN5;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=RNA polymerase sigma factor RpoH {ECO:0000256|HAMAP-Rule:MF_00961};
DE AltName: Full=RNA polymerase sigma-32 factor {ECO:0000256|HAMAP-Rule:MF_00961};
GN Name=rpoH {ECO:0000256|HAMAP-Rule:MF_00961};
GN ORFNames=B7Y41_04295 {ECO:0000313|EMBL:OYY94798.1};
OS Hydrogenophilales bacterium 28-61-23.
OC Bacteria; Pseudomonadota; Hydrogenophilia; Hydrogenophilales.
OX NCBI_TaxID=1970530 {ECO:0000313|EMBL:OYY94798.1, ECO:0000313|Proteomes:UP000215898};
RN [1] {ECO:0000313|EMBL:OYY94798.1, ECO:0000313|Proteomes:UP000215898}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=28-61-23 {ECO:0000313|EMBL:OYY94798.1};
RA Kantor R.S., Colenbrander Nelson T., Marshall S., Bennett D., Apte S.,
RA Camacho D., Thomas B.C., Warren L.A., Banfield J.F.;
RT "Lifting the veil on microbial sulfur biogeochemistry in mining
RT wastewaters.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is involved in regulation of expression of
CC heat shock genes. {ECO:0000256|HAMAP-Rule:MF_00961}.
CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme.
CC {ECO:0000256|HAMAP-Rule:MF_00961}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00961}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoH subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00961}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYY94798.1}.
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DR EMBL; NCGJ01000003; OYY94798.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A258TTN5; -.
DR Proteomes; UP000215898; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0009408; P:response to heat; IEA:UniProtKB-UniRule.
DR CDD; cd06171; Sigma70_r4; 1.
DR Gene3D; 1.10.601.10; RNA Polymerase Primary Sigma Factor; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_00961; Sigma70_RpoH; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012759; RNA_pol_sigma_RpoH_proteobac.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; TIGR02392; rpoH_proteo; 1.
DR NCBIfam; TIGR02937; sigma70-ECF; 1.
DR PANTHER; PTHR30376:SF3; RNA POLYMERASE SIGMA FACTOR RPOH; 1.
DR PANTHER; PTHR30376; SIGMA FACTOR RPOH HEAT SHOCK RELATED; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00961};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00961};
KW Sigma factor {ECO:0000256|ARBA:ARBA00023082, ECO:0000256|HAMAP-
KW Rule:MF_00961};
KW Stress response {ECO:0000256|ARBA:ARBA00023016, ECO:0000256|HAMAP-
KW Rule:MF_00961};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00961};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00961}.
FT DOMAIN 78..91
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00715"
FT DOMAIN 251..277
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00716"
FT DNA_BIND 252..271
FT /note="H-T-H motif"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00961"
FT REGION 54..123
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00961"
FT REGION 227..279
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00961"
FT MOTIF 78..81
FT /note="Interaction with polymerase core subunit RpoC"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00961"
SQ SEQUENCE 283 AA; 31663 MW; 887A85D43F99128B CRC64;
MTTQTLNFPV PNVLGSLENY IQAVNRFPML SAEQEHELAV RFRDQEDLEA ARQLILSHLR
LVVAIARGYL GYGLPHADLI QEGNIGLMKA VKRYDPERGV RLVSFAVHWI KAEIHEFVLK
NWRLVKVATT KAQRKLFFNL RSMKTGSSSL SASEVEAMAK TLNVNQSDVR EMETRLYGQD
MALEGSMDDD DDAFAPIAYL AAVDAEPTQH IEAAEREKLA SVGLSQALGS LDARSRHIIE
ARWLNEENPA TLHELAAVYS ISAERVRQVE AKALQKLKAV LTV
//
