ID A0A261FY44_9BIFI Unreviewed; 757 AA.
AC A0A261FY44;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=BE0216_07905 {ECO:0000313|EMBL:QOL32385.1}, BEUL_2284
GN {ECO:0000313|EMBL:OZG64018.1};
OS Bifidobacterium eulemuris.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=1765219 {ECO:0000313|EMBL:OZG64018.1, ECO:0000313|Proteomes:UP000216057};
RN [1] {ECO:0000313|EMBL:OZG64018.1, ECO:0000313|Proteomes:UP000216057}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 100216 {ECO:0000313|EMBL:OZG64018.1,
RC ECO:0000313|Proteomes:UP000216057};
RX PubMed=28764658; DOI=10.1186/s12864-017-3955-4;
RA Lugli G.A., Milani C., Turroni F., Duranti S., Mancabelli L.,
RA Mangifesta M., Ferrario C., Modesto M., Mattarelli P., Jiri K.,
RA van Sinderen D., Ventura M.;
RT "Comparative genomic and phylogenomic analyses of the Bifidobacteriaceae
RT family.";
RL BMC Genomics 18:568-568(2017).
RN [2] {ECO:0000313|EMBL:QOL32385.1, ECO:0000313|Proteomes:UP000593943}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 100216 {ECO:0000313|EMBL:QOL32385.1,
RC ECO:0000313|Proteomes:UP000593943};
RA Kim J.;
RT "Genome sequencing of Bifidobacterium eulemuris_DSMZ_100216.";
RL Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
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DR EMBL; MWWZ01000019; OZG64018.1; -; Genomic_DNA.
DR EMBL; CP062938; QOL32385.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A261FY44; -.
DR KEGG; beu:BE0216_07905; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000216057; Unassembled WGS sequence.
DR Proteomes; UP000593943; Chromosome.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536}.
FT DOMAIN 40..288
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 660..746
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 490
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 556
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 757 AA; 83508 MW; DD29A6650D9B3A4C CRC64;
MSHITRIQGA SSNGTALTAA YVEQTDANIA FALVSPDGEL PRLVHWGRPL ADPETVIALY
DALKPQRVSG ALDDVAWPSI LPTQAESWIG EPRLVLRRDG VEMFCKFTVA DIAVADDMGT
LDAPWEFNAD GSHVAPAQPV PGVRVIARDD EQGVEIAWRM ELLPGGLIRQ QAAVRNIAEE
AAPLEVGHVE LGFPLPAQMS EILTTTGRHL RERSPQRQPL TIGRFAKSSL VGRPDFDASL
LLSVGVPGFG FEHGEVYSVH VGWSGNSVLS AERLPYTQGV IGGGELLFGG EIVLGGADDV
YTTPWVYGSY GDGLNEVSAR FHDHLRSRHP RLIEKPRPVI LNTWEAVYFD HNFDTLAALA
DKAVESGVER FVVDDGWFGS RRDDTSGLGD WQISPEVWPD GDKSLKALAD YVHGKGMEFG
LWFEPEMINP DSDVARAHPD WVLAPTANRL PMQGRTQQVL DLTNPEAFDY ILGCMDQLVG
ELGIDYIKWD HNKLVTEAAS RSTGRPAVHQ QTEAVYEIFR ELIQRHPGLE IESCSSGGGR
VDLGILEYAS RIWVSDCVDP VERADIQRYT SLLVPPMMMG EHVGASPAHS TKRSTSQEMR
MATAFFGHMG IEWNLNKEPQ EALDLLGAWV SEFKKHRGWF AQGDCVHADS ADQSVRLDGM
VKPDRSAAVY RFTQLTTSQT YPSAPVHLPG LDPRETYRVL PLGTRRQSNP EIHCGQSELG
WWNDNGVVLP GEILRTYGIR PPAIQPEEAV LFYVVKA
//
