ID A0A265NGN2_9BACI Unreviewed; 280 AA.
AC A0A265NGN2;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE SubName: Full=2,5-diketo-D-gluconic acid reductase {ECO:0000313|EMBL:OZU90584.1};
GN ORFNames=CIL03_05435 {ECO:0000313|EMBL:OZU90584.1};
OS Virgibacillus indicus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Virgibacillus.
OX NCBI_TaxID=2024554 {ECO:0000313|EMBL:OZU90584.1, ECO:0000313|Proteomes:UP000216498};
RN [1] {ECO:0000313|EMBL:OZU90584.1, ECO:0000313|Proteomes:UP000216498}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IO3-P2-C2 {ECO:0000313|EMBL:OZU90584.1,
RC ECO:0000313|Proteomes:UP000216498};
RA Xu B., Hu B., Wang J., Zhu Y., Huang L., Du W., Huang Y.;
RT "Virgibacillus indicus sp. nov. and Virgibacillus profoundi sp. nov, two
RT moderately halophilic bacteria isolated from marine sediment by using the
RT Microfluidic Streak Plate.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OZU90584.1}.
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DR EMBL; NPMS01000001; OZU90584.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A265NGN2; -.
DR OrthoDB; 9804790at2; -.
DR Proteomes; UP000216498; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19132; AKR_AKR5D1_E1; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 2.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000216498}.
FT DOMAIN 19..262
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 52
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 110
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 77
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 280 AA; 32180 MW; 7B666BC4C5899B5C CRC64;
MNLSIPEITL NDGTTLPAIG FGTYNLNGNA GVNALTSAID TGYRLLDTAY NYENEGTVGE
AVRRSSIPRE QLKITSKLPG RYQEYDLAVT AIQESLFRAG LDYYDLYLIH WPNPKQDHYA
EAWQALIDAR KWGLIRSIGV CNFLPEHLER LEKETGVKPS INQVELHPFF NQEERRKWHQ
ENDIQTQSWS PLVRANEVME NETLTKMAEH HQKTVSQVIL RWHHQLGSIP IPKSSSSKRQ
LENISIFDFF LDETEMTQIS ALSRADGRMK DQDPAIYEEF
//
