ID   A0A265Q157_9FIRM        Unreviewed;       339 AA.
AC   A0A265Q157;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   24-JAN-2024, entry version 15.
DE   SubName: Full=Aminopeptidase {ECO:0000313|EMBL:OZV10243.1};
GN   ORFNames=CIW83_21335 {ECO:0000313|EMBL:OZV10243.1};
OS   Tissierella sp. P1.
OC   Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae;
OC   Tissierella.
OX   NCBI_TaxID=1280483 {ECO:0000313|EMBL:OZV10243.1, ECO:0000313|Proteomes:UP000216906};
RN   [1] {ECO:0000313|EMBL:OZV10243.1, ECO:0000313|Proteomes:UP000216906}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=P1 {ECO:0000313|EMBL:OZV10243.1,
RC   ECO:0000313|Proteomes:UP000216906};
RA   Bukhtiyarova P.A., Antsiferov D.V., Avakyan M.R., Frank Y.A., Ikkert O.P.,
RA   Pimenov N.V., Brasseur G., Tuovinen O.H., Karnachuk O.V.;
RT   "Isolation and Characterization of an Anaerobic Sulfidogenic Tissierella
RT   from Cu-bearing Coins.";
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OZV10243.1}.
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DR   EMBL; NPMN01000058; OZV10243.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A265Q157; -.
DR   OrthoDB; 9772053at2; -.
DR   Proteomes; UP000216906; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05656; M42_Frv; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF5; ENDOGLUCANASE; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438,
KW   ECO:0000313|EMBL:OZV10243.1}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000216906}.
FT   ACT_SITE        198
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         60
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         170
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         170
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         199
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         221
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         309
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   339 AA;  37743 MW;  7FA48CA00B4B0106 CRC64;
     MLLKQLTELP GVSGNEKEVR DFIISEIKDY VDSFKIDRIG NIIAYKKGKD TNKTLMVTAH
     MDEVGLLVKE IDNMGLLKFT TVGGIDKRIL VSKPVLVGKD KIMGVIGAKP IHLQKREEWM
     RALSIDELYI DIGAKSKEEA EKLVSIGDYV SFASDYVEFG ESLVKAKALD NRVGCSMLVN
     LIKEVKDVSF YAVFTVMEEV GLVGAGPAAY EVNPDISIVL EGTLCYDMPK LDTHLVPTYL
     NKGPAISLID RTTVFNHEFR KKIVSIAEKN NIPYQYRKTS MGGNDSGKIH TTKEGSITTT
     ISVPCRYIHS PASVMSKNDY GNTYLLLKAI LSEFEREEL
//