ID A0A267DE24_9PLAT Unreviewed; 1055 AA.
AC A0A267DE24;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 28-JUN-2023, entry version 19.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891, ECO:0000256|RuleBase:RU362092};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891, ECO:0000256|RuleBase:RU362092};
GN ORFNames=BOX15_Mlig014717g3 {ECO:0000313|EMBL:PAA46937.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA46937.1, ECO:0000313|Proteomes:UP000215902};
RN [1] {ECO:0000313|EMBL:PAA46937.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA46937.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA46937.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Introduces a single-strand break via transesterification at a
CC target site in duplex DNA. Releases the supercoiling and torsional
CC tension of DNA introduced during the DNA replication and transcription
CC by transiently cleaving and rejoining one strand of the DNA duplex. The
CC scissile phosphodiester is attacked by the catalytic tyrosine of the
CC enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme
CC intermediate and the expulsion of a 3'-OH DNA strand.
CC {ECO:0000256|RuleBase:RU362092}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213,
CC ECO:0000256|RuleBase:RU362092};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446, ECO:0000256|RuleBase:RU362092}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAA46937.1}.
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DR EMBL; NIVC01004644; PAA46937.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A267DE24; -.
DR STRING; 282301.A0A267DE24; -.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR013498; Topo_IA_Znf.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR010666; Znf_GRF.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF01396; zf-C4_Topoisom; 1.
DR Pfam; PF06839; zf-GRF; 2.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
DR PROSITE; PS51999; ZF_GRF; 2.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362092};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU362092};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029,
KW ECO:0000256|RuleBase:RU362092}; Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00047}.
FT DOMAIN 1..122
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT DOMAIN 780..819
FT /note="GRF-type"
FT /evidence="ECO:0000259|PROSITE:PS51999"
FT DOMAIN 917..959
FT /note="GRF-type"
FT /evidence="ECO:0000259|PROSITE:PS51999"
FT DOMAIN 1035..1050
FT /note="CCHC-type"
FT /evidence="ECO:0000259|PROSITE:PS50158"
FT REGION 341..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 757..779
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 953..1042
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1055 AA; 111804 MW; F88A82C155948D70 CRC64;
MRRRDGQSRY NKIYEFQCNL LGQQCQMTMT SVSGHLMNYD FTQQHRKWLS CHPSALFDAP
VVKQIQRDYQ PIRDTLTQLA KSAQRLVIWT DCDREGENIG MEIVNVCMAS NPRLDVYRAK
FSEITRPAVQ RALASLTRMD ERVSDAVDAR QELDLRIGAA FTRFQTLRLR QVFPAVLADQ
LVSYGSCQFP TLGFVVERFK EAERFQPEPF WRLRVLAESP DTGQSVEFLW KRGRVYDQAV
GQMFLAHVLE SPIATVISVE SKPKNRWRPC ALDTVEFEKL ASRKLRINAK EAMHIAERLY
TQGYISYPRT ETNIFPRDFN LRGLVQTQTA DNRWAQFAQR VAEHGPSPRN GKKSDQAHPP
IHPLKCGDGL ANNEAKVFEL IARHFLACCS KDAVGREVKV GIDINGEGFE AKGLTIIERN
YLEVYIYDKW VDKELPLFQQ GQQFQPSNIS LIDGCTTAPA LLTEADLIAL MDRHGIGTDA
THAEHIETVK QRLYVGLQDA RYLVPGQLGI GLVEGYDAMG FQMSKPDLRS ALEADLKAIC
EGRRSKQDVL SFHIERYRRV FDEACRQANL LDARVGHYLD EQPAEAPQLP AGEAGGQANS
TNLVRRCPQC SAADMALKTK RDGSSYYVGC LAYPTCKRAV WLPESVRRAA VSDQNCPTCS
TPGGADGQVR KLTLQFSQAA PMPFRDLAEH TICVFCDPDY VGAFGGAAST VGGGGGGGQP
KPSTAISFHA NELQAASMQQ RLFLQPQDRQ TFAVQAPAPM QQQPPPQPPG GASNAAPGTC
ACGTPVRELT VRKEGPNKGR QFYKCATGTC NHFQWADAPP GGGGGGGGGF AGASFSASST
QYGGGGDFGS GGGGGFGGNS GFGGGGGGGF GGGGGGGSSG FGGGGGGGFG GGGGGGSSGF
GGGGGGSGGG AASGVSCKCG EPARERTVQK DGPNKGRPFF GCAKPMNQSC NFFQWGDEPA
GGGGGGGGGG GSGFANSSFG SGSSRGGFSR GGGGGGGGGG GFQKANSHGN RRSGGGGGGG
GGSDGGGGDG QKKQRKCGAC GQPGHTRNRC AMFIN
//
