ID A0A267DE36_9PLAT Unreviewed; 761 AA.
AC A0A267DE36;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Calpain catalytic domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=BOX15_Mlig013791g4 {ECO:0000313|EMBL:PAA47560.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA47560.1, ECO:0000313|Proteomes:UP000215902};
RN [1] {ECO:0000313|EMBL:PAA47560.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA47560.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA47560.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase C2 family.
CC {ECO:0000256|ARBA:ARBA00007623}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAA47560.1}.
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DR EMBL; NIVC01004409; PAA47560.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A267DE36; -.
DR STRING; 282301.A0A267DE36; -.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00214; Calpain_III; 1.
DR CDD; cd00044; CysPc; 1.
DR CDD; cd16182; EFh_PEF_Group_II_CAPN_like; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR033883; C2_III.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR022683; Calpain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR10183; CALPAIN; 1.
DR PANTHER; PTHR10183:SF379; CALPAIN-A-RELATED; 1.
DR Pfam; PF01067; Calpain_III; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00720; calpain_III; 1.
DR SMART; SM00230; CysPc; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00239};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU00239}; Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807, ECO:0000256|PROSITE-
KW ProRule:PRU00239}.
FT DOMAIN 105..398
FT /note="Calpain catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50203"
FT DOMAIN 661..696
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT REGION 69..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 159
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 313
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 338
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
SQ SEQUENCE 761 AA; 87190 MW; 4A7B9DCEB9D2D8BF CRC64;
MPRVQIVYDT SGSTSLWSGG NNPFYDPYYK GYTFDADQSN NGGGRNIFDY LDRRSKSVVI
DGDAFVPRAL KDPGPPRTIY RQAGSDRPKV DFNSKRDEQK LSGRLFEDPD FPADNRSIYP
SRSYSRFEWK RPKEICSDPL FISDGVSRFD VKQGELGDCW LLAAVASLSM HKKLFDQVVV
PDQDFGANYC GMFRFRFWQF GEWKDVEIDD RLPTKYGKLC FMRSEDRNEF WSALLEKAYA
KLNGSYEALS GGNQAEAMVD FTGGLCETFD MRRAPPNLYN IMVQANNACS LMGCAIESEQ
LEGHLSNGLI TGHAYSVTDV KKVRLNNGME ESLVRVRNPW GNECEWKGDW SDHSHKWSSV
SQGEKDKLGM DQRDDGEFWM SYKDFSSQFN KIEICHLGPE SMSSSVKGEA AKKRRWEMCL
ESGQWKRNVS AGGCRNNIDS FHMNEQFRIE IVDPDEDDDE NMGTIIVGLM QKDIRKILKG
ADFLTIGYAI YSIKDPNIPP LDQKFFRFNR QVDKSPSFVN QREVCGRHKL PPGHYVIIPS
TFSPNEEAEF ILRIFSEKPN ESGVLDTPNT MDNVVDGVYG RKHHSTVMEN EEPLKKAFME
FAGSTAKIHA KQLQQILNRS FGREIKEFQG FSLEACRSML ALVDANMSGF MEFDEFKHLW
NEIRLLKTIF KEYDADGNGY FDSFELRGAL RVAGYNLCNK VFESVAMRYM DPEQGRVCFE
DFILLSIRLK TAFETYKAMP KTNEGFGVVK QDHFLLQTLY L
//
