ID A0A267DRW4_9PLAT Unreviewed; 803 AA.
AC A0A267DRW4;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=PDEase domain-containing protein {ECO:0000259|PROSITE:PS51845};
GN ORFNames=BOX15_Mlig007514g1 {ECO:0000313|EMBL:PAA51955.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA51955.1, ECO:0000313|Proteomes:UP000215902};
RN [1] {ECO:0000313|EMBL:PAA51955.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA51955.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA51955.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAA51955.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; NIVC01003329; PAA51955.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A267DRW4; -.
DR STRING; 282301.A0A267DRW4; -.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0004114; F:3',5'-cyclic-nucleotide phosphodiesterase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR CDD; cd00077; HDc; 1.
DR Gene3D; 3.30.450.40; -; 2.
DR Gene3D; 1.10.1300.10; 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR023088; PDEase.
DR InterPro; IPR002073; PDEase_catalytic_dom.
DR InterPro; IPR036971; PDEase_catalytic_dom_sf.
DR PANTHER; PTHR11347; CYCLIC NUCLEOTIDE PHOSPHODIESTERASE; 1.
DR PANTHER; PTHR11347:SF221; DUAL 3',5'-CYCLIC-AMP AND -GMP PHOSPHODIESTERASE 11; 1.
DR Pfam; PF01590; GAF; 2.
DR Pfam; PF00233; PDEase_I; 1.
DR PRINTS; PR00387; PDIESTERASE1.
DR SMART; SM00065; GAF; 2.
DR SMART; SM00471; HDc; 1.
DR SUPFAM; SSF55781; GAF domain-like; 2.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS51845; PDEASE_I_2; 1.
PE 4: Predicted;
KW cGMP {ECO:0000256|ARBA:ARBA00022535};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR623088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902}.
FT DOMAIN 473..795
FT /note="PDEase"
FT /evidence="ECO:0000259|PROSITE:PS51845"
FT ACT_SITE 549
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-1"
FT BINDING 553
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 590
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 591
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 591
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
FT BINDING 701
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR623088-3"
SQ SEQUENCE 803 AA; 87930 MW; 4F423F191DEE4B3B CRC64;
MSEAEEAAVE AWLDSHPDFF EDYFHRRGSR GLVDGWLRRR LAATSSAALG TVQLAPRSLS
CSVGSGRLLG QRSHRIRKAS SELHADEGSI GRTSPAMPRR HALPALTEEA ATAREMMLSS
LLMELDSGRL RRRIVRACRL LLAADRCALF LAEPGGWLRL TADTVAAEAA PTEPEVRVPP
GVGIVGNAAA TGQTLRLEEA AKDPRFNAAV DELPSCATVR ALAAPLKNPD GGSIGLLLAT
SGGRSAAFTD AHEKSLQTFL ALCSASLRNS ELFQQSQLET KRNEVLLDLV RITFEHMENL
ELLVNNILQY MRLITRCTWC QILLIDDPSS AEGSFDRVFE LLADEADGTD KSAGGGRLLI
SSEIAQSVLS SGEPLIVPRA ESDSRLTPAE RSRIRSLLCL PVRNALSEPS GLCLLMNPAD
GRAFSESDAN LAEAFALFCG LGIHSVRQRE LAETAQKRQR VALEVLGYHA AAGQEEAQRL
ARSLVPSARY FGIDQLAFDE FSVPEGDTLL CSIKLFQEAG LLAALQLDYR ALCLWLLSVH
KNYRPLLHHN WRHAFGVAHC ACRLAKQSPE LSSHLSPLEQ LAVLVAALCH DLDHQDPDGF
GQRAGLDPLA ELYSSSVTER RHFSRCIMLL SLEGCDILAR LPQAQYSACI SLVERCILAT
DLRRHFESRP GLQRQAQTGR VDWSSKPQRD LLLGLVMTCS DLGTFAKPWP QHRRVAQLVT
DQYRQLSGRA KVESSENAKD QSHDLSLLAE QHVAFIKSVC LPAFEMLTSL CDNLEELRSG
CQANLAEWAA LAAGNEQPQA DSK
//
