ID A0A267ENM7_9PLAT Unreviewed; 1784 AA.
AC A0A267ENM7;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU366018};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU366018};
DE Flags: Fragment;
GN ORFNames=BOX15_Mlig005236g1 {ECO:0000313|EMBL:PAA63098.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA63098.1, ECO:0000313|Proteomes:UP000215902};
RN [1] {ECO:0000313|EMBL:PAA63098.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA63098.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA63098.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Ubiquitin ligase protein which is a component of the N-end
CC rule pathway. Recognizes and binds to proteins bearing specific N-
CC terminal residues that are destabilizing according to the N-end rule,
CC leading to their ubiquitination and subsequent degradation.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU366018};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU366018}.
CC -!- SIMILARITY: Belongs to the UBR1 family. {ECO:0000256|ARBA:ARBA00009750,
CC ECO:0000256|RuleBase:RU366018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAA63098.1}.
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DR EMBL; NIVC01001873; PAA63098.1; -; Genomic_DNA.
DR STRING; 282301.A0A267ENM7; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR GO; GO:0071596; P:ubiquitin-dependent protein catabolic process via the N-end rule pathway; IEA:UniProtKB-UniRule.
DR CDD; cd19672; UBR-box_UBR1_like; 1.
DR Gene3D; 2.10.110.30; -; 1.
DR Gene3D; 3.30.1390.10; -; 1.
DR InterPro; IPR003769; ClpS_core.
DR InterPro; IPR044046; E3_ligase_UBR-like_C.
DR InterPro; IPR014719; Ribosomal_bL12_C/ClpS-like.
DR InterPro; IPR039164; UBR1-like.
DR InterPro; IPR003126; Znf_UBR.
DR PANTHER; PTHR21497:SF24; E3 UBIQUITIN-PROTEIN LIGASE UBR1; 1.
DR PANTHER; PTHR21497; UBIQUITIN LIGASE E3 ALPHA-RELATED; 1.
DR Pfam; PF02617; ClpS; 1.
DR Pfam; PF18995; PRT6_C; 1.
DR Pfam; PF02207; zf-UBR; 1.
DR SMART; SM00396; ZnF_UBR1; 1.
DR SUPFAM; SSF54736; ClpS-like; 1.
DR PROSITE; PS51157; ZF_UBR; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU366018};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU366018};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366018};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366018};
KW Zinc-finger {ECO:0000256|RuleBase:RU366018}.
FT DOMAIN 75..147
FT /note="UBR-type"
FT /evidence="ECO:0000259|PROSITE:PS51157"
FT ZN_FING 75..147
FT /note="UBR-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00508"
FT REGION 1033..1077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1104..1139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1059..1077
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1108..1124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:PAA63098.1"
SQ SEQUENCE 1784 AA; 197109 MW; A2FA2E40799033BD CRC64;
RNLQSYYQAG QLGEGLKQLF FNSGPKVFAP KVRPDLLFLD VLARNQILSP LEQFITASAT
PDVEFEKLTQ DKNPGICGYI LKNNEPVFTC HDCTADPTSV MCNNCFVHSE HKNHNYKMHL
SSGNGGYCDC GDPEAFKRYH RCSLHERQAG SQSGAPVSPV EKLPAGMADR FRAICNQVLQ
LALHLTLLEY RIRLPSNVDR LDEVDLNLLR RWLPHGERTD TSESDAEAGG QYASVLYNDE
VHSYPQVINT LTQCLGVDEH DANHFATVVD NDGRSVVSLS DWASAKKLCS EIRGTIRESS
GFELGCYPVR SVSFAYQSMC LYLLSWLGQL IKQSDGLHEL FCQSLLAPVD NFPGTEWAPG
QPLVYYLLVH TDHLWKAARK SFIEMLIKSL LMKANYKRQI AALYAVNQEQ IFERFCVDMH
HQEDSIAQLS VQLFTSPSLA MYLITEQKAL KILVDILIKS VLQRSVQQHQ ESGRQLLKFS
EDDRMPFSRW NRSLHLVRDI EYLLHNDFEQ LEPNFFAAIN EALPSFLQVY SLFQDTCCAR
RYIVGHIGFD FEWEMPFIFF LSSMYQNHLL AEFLSRDENV FSTILDKVLD AIEQKPPLSA
NFLADSGQRV RLAGHVLSGR ALAPLMSGRV FISHFQPLVR FLDSLLACTM ANHEASPQCR
RQLELLANRP GFEIAWLTSA RQLAWSAQIA AGLWKRNGLA LMNVNLQYSS HQSAAVGMSL
RDLHLVQLAA ATVPPDQFIL RLLDAFELQR WTLGAASSSE SAAERYDNEA ERSIVEEMLR
CLINVVCERR VPGVGQSVAF MEMVEEDLLQ HLVAEPQSHS KLLRLANDLV RKLPAADRQL
ECFAKLDGVI DKLAEFRGVQ GQNKGEFHLR EQHYSRFNPF YPRLAKSATS KALHSMTKRR
AATPAADLPI VRPPQLPAFA AAYQPIVELL HCDVMLGLMR AVVQATKDAG ASSKRHWSEV
QLERVLYLML VGLDEDQRRG DRRFLDCVLS GIGEASLMSA MESVPKSRLA MDSVRQLHGY
TLARLRRLCT GQGEGGESGG NEAGEVGGGG SGGLDAARDA AEAEVRRRQQ QKLAQDRRDR
LMAKMMRMQK NFLAEYGDLV EQADDKSGGA TASENSTEST SAGRRSDGPL IGPNRTVAHE
PQPGLTCLLC QEANRPDSRD PMLLAGLVQR STVLSQDRGR SRDTDWSGCF VEAELLEGSH
FSSCGHAMHR SCYSDFMDSP SNVHVNRGGE LGRSRYLCPL CQQLCNCALP VAPSLPCSAE
QQQQHQFDAN ADSGRFDSWL SSIRDGVAAG LPKWPPQAAA ESRVVAFRKN HDDNSWPGAE
AAQALNNLAK VCEVRGLGAD LPPELMPTGW KLPEKADPLL LTVAFSLQAA ETAGRATERP
LFSALPERCA VGLAALVRAV AQRPVASDSD FDRLHQLAIN NLSLLLHLQR PNLPLPCLLE
ADMSTCLVQL TYCLYRMAGQ PPDCSDADLC RLIRLVAGAH VFQICLTLPS DPDASTNADS
DADASGDDSD CRKLDRLVRQ CRSAVGLPPA ALSGPGLADQ VAAACLPLLR CAAIFYRALT
DAPAPSEPEP ASSAEAEFAW LAGLLQLPRR PCDLPTEQLV SLWCQDSARL QARAASPAVR
YPRQAARLVS LPTDYTDLLL ECSDFQCPSG AGPGPAGRSL QPAKCLACGK LVCSNSNCCQ
MPVFNGDRAI GGANLHAVMC TGGTGVFLRV LDCTLLLVSA PNRLGCISSA PYVDRYGETD
EYLRRGQPLQ LSSAMYSRLQ EDWLRHSLAA RAGRDPHSGN FFEV
//
