ID   A0A267GEE4_9PLAT        Unreviewed;       684 AA.
AC   A0A267GEE4;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:PAA83669.1};
GN   ORFNames=BOX15_Mlig019851g3 {ECO:0000313|EMBL:PAA83669.1};
OS   Macrostomum lignano.
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC   Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX   NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA83669.1, ECO:0000313|Proteomes:UP000215902};
RN   [1] {ECO:0000313|EMBL:PAA83669.1, ECO:0000313|Proteomes:UP000215902}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DV1 {ECO:0000313|EMBL:PAA83669.1};
RC   TISSUE=Whole organism {ECO:0000313|EMBL:PAA83669.1};
RA   Berezikov E.;
RT   "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT   model organism for stem cell research.";
RL   Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PAA83669.1}.
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DR   EMBL; NIVC01000412; PAA83669.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A267GEE4; -.
DR   STRING; 282301.A0A267GEE4; -.
DR   Proteomes; UP000215902; Unassembled WGS sequence.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR048912; BetaGal1-like_ABD1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21317; BetaGal_ABD_1; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           23..684
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012899119"
FT   DOMAIN          41..357
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          404..533
FT                   /note="Beta-galactosidase 1-like first all-beta"
FT                   /evidence="ECO:0000259|Pfam:PF21317"
FT   DOMAIN          560..619
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
FT   ACT_SITE        190
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        269
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   684 AA;  75930 MW;  04CA84729C2FEFE0 CRC64;
     MARKIDYSAL LFAAWFISFS IAVQHDSSVQ RSFIIDYDNN TFLKDGQPFQ YISGSIHYFR
     LLPDSWDDRL AKMRAAGLNA VQIYVPWNFH MPRPGQHRFT GSADLDLFLA LAQKHDLLAI
     VRAGPYICAE WDFGGLPAWL LQRNASMVMR SSDPQFLAAV DEWMAVLLPI IARRLYSSGG
     NVIMVQLENE YGSTGQCDLA YIRHLYQLGR RYLGNQTVLF STDNTLTCVA KERQFYATID
     FGVDGTRSLK DTFAPLRALQ PKGPLVNSEY YAGWLDHWSS PHVRVGAQLV ADGLARMLAY
     GVNVNIYMFF GGTNFGYWNG ANSPPFEPQP TSYDYDAPIA ENGDTRYKYQ LIRQVIKDAT
     GRQPSPVPRN SSASSFGRVK LQRSASLLGN LDSLIVNRLD GKLPVTMESL GQFQGFALYG
     TQISEDFKAV APSLTSSYSL RVEKVRDFVY AFASSSSKPS LGQFLGFISY AESAAVLNFT
     ASDLSSIRNI WLLVENCGHV NSGPDIQGNV KGIIGQVTLN GAPLNGWRSY SLDFDRLAAK
     LPYAKRLAAS EEPQAAQASL YMGRLILDKN NLTDTYANLS TFCKGQLWIN GFNLGRYWPC
     AGPQVTLYVP RAVLREGSNS VAVLELLQAP CLSDSSCRID FLREPDVGFR SKSVADSAIS
     LRFRGKFAVP SSADQLPSVD DERI
//