ID A0A268JZC8_9BACI Unreviewed; 920 AA.
AC A0A268JZC8;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 14.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=CHI06_16440 {ECO:0000313|EMBL:PAE39721.1};
OS Bacillus sp. 7884-1.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=2021693 {ECO:0000313|EMBL:PAE39721.1, ECO:0000313|Proteomes:UP000216214};
RN [1] {ECO:0000313|EMBL:PAE39721.1, ECO:0000313|Proteomes:UP000216214}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=7884-1 {ECO:0000313|EMBL:PAE39721.1,
RC ECO:0000313|Proteomes:UP000216214};
RA Kalinowski J., Ahrens B., Al-Dilaimi A., Winkler A., Wibberg D.,
RA Schleenbecker U., Ruckert C., Wolfel R., Grass G.;
RT "Isolation and whole genome analysis of endospore-forming bacteria from
RT heroin.";
RL Submitted (JUL-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAE39721.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; NPDD01000136; PAE39721.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A268JZC8; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000216214; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Pyruvate {ECO:0000313|EMBL:PAE39721.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000216214}.
FT ACT_SITE 150
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
FT ACT_SITE 578
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ SEQUENCE 920 AA; 106202 MW; 17F3B2F74B4DD902 CRC64;
MNTELKMNDS SLPLRRDVKL LGQILGEILV NHGGTELLDK VEKIRMTCKT LRIHFDHEIY
AELKQEITNL SSPMRKQVIR AFSMYFHLIN IAEQNHRIRR KRQYHLQDDS IVQPDSIESA
ILSLKENNID ENMIQEVLNK LSLELVITAH PTEAAKRSIL EIQQRIAVIL KKLDQPLLTT
RERLKLEESL FNEVTILWQT DELRHTKPTV LDEVRNGLYY FDQTLFEVLP EIHREVEDCL
EKNFSTKNWE VPNFLKFGSW IGGDRDGNPN VTPEVTWETL HKQRKLVLRK YKNVLIDLMK
RYSHSTTRVE VSLELIQSLE EEENKYLTED KKWPITTEVY RRKFAIIIER LKQVGKSDLG
YRSSEELLED LFLVKRSLNK HHPAAHELKT IQKLIRQVQL FGFHLATLDI RNHSGEHEAA
ITEILRKVRI SDNYAELSED EKVRILENTL MDPRPLLLLN EDYTPETQQM IQVFQMIRNA
QKEFGVSSIS VYLVSMTKSP SDILEVLVLA KEAGIYRLHA DGTIENQLNV APLLETIDDL
TAGPKIMETL FKMPVYRNHL KVMNDQQEIM LGYSDGSKDG GTLTANWKLY KAQLEIHEMA
NKYHIGLKFF HGRGGSLGRG GGPLNKSLLS QPAETIGDGV KITEQGEVLS SRYLLEDIAY
RSLEQATSTL MLATAHVSKE AEHGNLRDES WELAIEEISS ISLKKYQSLV FGDSDFLTYF
NEATPLRELG DLNIGSRPMS RKNRGRFEDL RAIPWVFAWT QSRQLLPAWY AAGTGLESFA
SQNEQNLQQL QHMYEKWPFF RSTIDNLQMA LMKADITTAK EYLSLVEDQK IAERIFSNIL
EEYERTKAIL LKITGDKELL DHTPNIKDSV YRRNPYVDPL NFLQVELIKQ LRKQDEPSEE
LIIDVLLTIS GISAGLRNTG
//
