ID A0A285NIG5_9PSED Unreviewed; 944 AA.
AC A0A285NIG5;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=SAMN05660463_01120 {ECO:0000313|EMBL:SNZ08703.1};
OS Pseudomonas sp. URIL14HWK12:I9.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1261633 {ECO:0000313|EMBL:SNZ08703.1, ECO:0000313|Proteomes:UP000232228};
RN [1] {ECO:0000313|EMBL:SNZ08703.1, ECO:0000313|Proteomes:UP000232228}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=URIL14HWK12:I9 {ECO:0000313|EMBL:SNZ08703.1,
RC ECO:0000313|Proteomes:UP000232228};
RA Ehlers B., Leendertz F.H.;
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; OBEM01000002; SNZ08703.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A285NIG5; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000232228; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 1.
DR Gene3D; 3.30.950.30; Schlafen, AAA domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR007421; Schlafen_AlbA_2_dom.
DR InterPro; IPR038461; Schlafen_AlbA_2_dom_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF04326; SLFN_AlbA_2; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:SNZ08703.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000232228};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..141
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 156..453
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 797..929
FT /note="Schlafen AlbA-2"
FT /evidence="ECO:0000259|Pfam:PF04326"
SQ SEQUENCE 944 AA; 105891 MW; 7B09453B777F0B5D CRC64;
MNTENHSQTA AFLWSIADLL RGDFKQSQYG RIILPFTLLR RMECELAPTK DEVIKQTFAH
EGRPDTVREM FLRRATGHLQ FFNASPLTLA TLSNTQTAAD LTSYVQAFST DARDIFEHFH
FEGFVQQLAR ANLLYQVVQR FAATDLSPER ISNFGMGLIF EELIHKFSES SNETVGEHFT
PRDIARLTTS LVITEQDGKL APNSIVTIYD PTAGTGGFLS EGNGYIQSIS NKVTVSLYGQ
DINSESYAIC KANMLIKGQD ITSVKLGNTL SNDQLAEKSF DFMLSNPPFG VEWKKIQKQI
TDEYTEKGFD GRFGPGLPRV SDGSLLFLLH LVSKMRDPRD GGSRIGIILN GSPMFTGGAG
SGESEIRRYL LQNDLVEAVV ALPADMFYNT SIATYLLVMS NRKSADRIGK VQLIDASTIY
APMRKSIRNK RKFFAEEQVA EIIKLYEAFK DGSNSKILFN TELGYQSITI ERPLRLSFSV
TPTKLAAYER FKGSEQVELL GKVKGWFDNF PDFLIAAGIK SLTQGAEKAV LACFGERDPN
AKPMLKNNGE FIADIYGRKL VSVPLSQSVE DYFDREIRPY AEDSWLAPPS GDKTDRKIGI
IGYEINFNRF KVQGQIERLE SQYKSYSSSS LGHLAMAVID GGRVEQLLHR ENSLYFNKKG
SPRVKSVLNV DSKSHKDYIQ VVIGKEAVAD YIVTFFHSEL GALCLAACMQ GVTVKYFRSD
DLDRIVVALP SLDEQKEIVN TSRKLKSLKE AIERFDSEVA LNPTSSSSLN HRLDGMLEVI
GRLTVEDKIR NIIRMGETDV TELKSSFSLD IKKQEKADYI EDASLKTVVA FLNTDGGSLL
IGVSDDGELL GLNDEINRFH KGSKDKFLLY FKNKLKSRVG EQYYPFISTK LVILDGKNVL
WIDCKSSVNP CYLYGQDFYV RTNPATDKLE GPKLVEYVKN HFGY
//
