ID   A0A286DZ79_9ACTN        Unreviewed;       531 AA.
AC   A0A286DZ79;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   24-JAN-2024, entry version 16.
DE   RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE            EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN   ORFNames=SAMN06297387_11392 {ECO:0000313|EMBL:SOD63933.1};
OS   Streptomyces zhaozhouensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1300267 {ECO:0000313|EMBL:SOD63933.1, ECO:0000313|Proteomes:UP000219072};
RN   [1] {ECO:0000313|EMBL:SOD63933.1, ECO:0000313|Proteomes:UP000219072}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CGMCC 4.7095 {ECO:0000313|EMBL:SOD63933.1,
RC   ECO:0000313|Proteomes:UP000219072};
RA   Ehlers B., Leendertz F.H.;
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC         Evidence={ECO:0000256|ARBA:ARBA00001699,
CC         ECO:0000256|RuleBase:RU000555};
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
CC       {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; OCNE01000013; SOD63933.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A286DZ79; -.
DR   OrthoDB; 9768429at2; -.
DR   UniPathway; UPA00703; UER00720.
DR   Proteomes; UP000219072; Unassembled WGS sequence.
DR   GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   CDD; cd00727; malate_synt_A; 1.
DR   Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR   Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR   InterPro; IPR044856; Malate_synth_C_sf.
DR   InterPro; IPR011076; Malate_synth_sf.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR019830; Malate_synthase_CS.
DR   InterPro; IPR001465; Malate_synthase_TIM.
DR   InterPro; IPR048355; MS_C.
DR   InterPro; IPR048356; MS_N.
DR   InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR   NCBIfam; TIGR01344; malate_syn_A; 1.
DR   PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR   PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR   Pfam; PF20659; MS_C; 1.
DR   Pfam; PF20656; MS_N; 1.
DR   Pfam; PF01274; MS_TIM-barrel; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   SUPFAM; SSF51645; Malate synthase G; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW   ECO:0000256|RuleBase:RU000555};
KW   Reference proteome {ECO:0000313|Proteomes:UP000219072};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW   Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW   ECO:0000256|RuleBase:RU000555}.
FT   DOMAIN          12..65
FT                   /note="Malate synthase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF20656"
FT   DOMAIN          158..404
FT                   /note="Malate synthase TIM barrel"
FT                   /evidence="ECO:0000259|Pfam:PF01274"
FT   DOMAIN          411..529
FT                   /note="Malate synthase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF20659"
FT   ACT_SITE        162
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT   ACT_SITE        445
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ   SEQUENCE   531 AA;  59581 MW;  DB89D6B12DDF5FC5 CRC64;
     MSAVTVEARH RVPRQDEVLT PEALDFLAQL HQRFTPRRDE LLALRAERRA EIARTGRLDF
     LPGTAEVREG EWSVAPAPPA LQDRRVEITG PAERKMTVNA LNSGARVWLA DFEDALSPTW
     ENVIGGQLSL ADAYRRRIDF TDERGKRYAL LPDAELATVV ARPRGWHLDE RHLTVGGREI
     PGALVDFGLY FFHNARWLMD MGKGPYFYLA KLESHLEARL WNDVFVFAQE TLGVPHGSVR
     ATVLIETVTA AFEMEEILYE LRDHASGLNA GRWDYLFSIV KNFRDGGEGY VLPDRNAVTM
     TAPFMRAYTE LLVRTCHKRG AHAIGGMAAF IPSRDAEVNK VAFEKVRTDK EREAADGFDG
     SWVAHPGLVP VAEGAFDAVL GARPHQKDRL REEVRVTAEQ LLDIGSVPAR PTVEGLRNAV
     QVGTRYLQSW LAGRGAVAVF HLMEDVATAE ISRSQIWQWI NAGVLLDNGE RVTHSYVRQI
     AADQLVQIRD EVGEDAYVSG GWERAFDLLM EIALDESYVD FLTLPAYRLL D
//