ID   A0A286ET27_9ACTN        Unreviewed;       456 AA.
AC   A0A286ET27;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=tRNA/tmRNA/rRNA uracil-C5-methylase (TrmA/RlmC/RlmD family) {ECO:0000313|EMBL:SOD74077.1};
GN   ORFNames=SAMN05892883_3253 {ECO:0000313|EMBL:SOD74077.1};
OS   Jatrophihabitans sp. GAS493.
OC   Bacteria; Actinomycetota; Actinomycetes; Jatrophihabitantales;
OC   Jatrophihabitantaceae; Jatrophihabitans.
OX   NCBI_TaxID=1907575 {ECO:0000313|EMBL:SOD74077.1, ECO:0000313|Proteomes:UP000218903};
RN   [1] {ECO:0000313|EMBL:SOD74077.1, ECO:0000313|Proteomes:UP000218903}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GAS493 {ECO:0000313|EMBL:SOD74077.1,
RC   ECO:0000313|Proteomes:UP000218903};
RA   Ehlers B., Leendertz F.H.;
RL   Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LT907982; SOD74077.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A286ET27; -.
DR   InParanoid; A0A286ET27; -.
DR   Proteomes; UP000218903; Chromosome i.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          17..80
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   REGION          214..253
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        396
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         272
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         301
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         325
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         369
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   456 AA;  48214 MW;  93B06DF5779BEF15 CRC64;
     MPTDPRTSTG RDDVTAPFAV GHRLRLRVTA VAHGGHCVAR VDDDPHGRVV FVRHTLPGEV
     VEAAVTEDSG GAFLRADAVE ILQAATERVA PPCPHAGAGR CGGCDWQHVT AGGQRELKRA
     VVGEQFARLA QLEVDAEVEE LAGGLLGWRT RTLYATTPGG EVGLRRHRSH TVEVLDHCPL
     GVHGVGDSEV LQEPWPGISG LELVQDGPQA ITLLTHRPAP RPSGRRGSTQ RGGGQRHHRR
     QPDVVSVHSG SPEVTRPALG RSFSVAAAGF WQVHPLAAET FARAMLDGLA PAAGEVVLDL
     YAGAGLFTAL IGERVGLTGR VIGLEADRQA VADAETNLAD LPWCSVRQGK VSAELIADVG
     AGCDLVVLDP PRSGAGREIM LAALQLRPRL IGYLACDPSS LARDVATARE AGWELRQLRA
     FDAFPMTHHL ECLAVLAPTP TGEHATSATG RRTPVG
//