ID A0A286ULH3_9AGAM Unreviewed; 544 AA.
AC A0A286ULH3;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Cysteine proteinase 1, mitochondrial {ECO:0000256|ARBA:ARBA00016900};
DE EC=3.4.22.40 {ECO:0000256|ARBA:ARBA00012465};
DE AltName: Full=Bleomycin hydrolase {ECO:0000256|ARBA:ARBA00030627};
DE AltName: Full=Homocysteine-thiolactonase {ECO:0000256|ARBA:ARBA00032353};
DE AltName: Full=Leucine aminopeptidase 3 {ECO:0000256|ARBA:ARBA00031564};
DE AltName: Full=Y3 {ECO:0000256|ARBA:ARBA00031859};
GN ORFNames=PNOK_0293200 {ECO:0000313|EMBL:PAV20305.1};
OS Pyrrhoderma noxium.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Hymenochaetales; Hymenochaetaceae; Pyrrhoderma.
OX NCBI_TaxID=2282107 {ECO:0000313|EMBL:PAV20305.1, ECO:0000313|Proteomes:UP000217199};
RN [1] {ECO:0000313|Proteomes:UP000217199}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FFPRI411160 {ECO:0000313|Proteomes:UP000217199};
RA Chung C.-L., Lee J.T., Akiba M., Lee H.-H., Kuo T.-H., Liu D., Ke H.-M.,
RA Yokoi T., Roa M.B., Lu M.J., Chang Y.-Y., Ann P.-J., Tsai J.-N.,
RA Chen C.-Y., Tzean S.-S., Ota Y., Hattori T., Sahashi N., Liou R.-F.,
RA Kikuchi T., Tsai I.J.;
RT "The Genomic Landscape Of Tree Rot In Phellinus noxius And Its
RT Hymenochaetales Members.";
RL bioRxiv 0:0-0(2017).
CC -!- FUNCTION: The normal physiological role of the enzyme is unknown, but
CC it is not essential for the viability of yeast cells. Has
CC aminopeptidase activity, shortening substrate peptides sequentially by
CC 1 amino acid. Has bleomycin hydrolase activity, which can protect the
CC cell from the toxic effects of bleomycin. Has homocysteine-
CC thiolactonase activity, protecting the cell against homocysteine
CC toxicity. Acts as a repressor in the GAL4 regulatory system, but this
CC does not require either the peptidase or nucleic acid-binding
CC activities. {ECO:0000256|ARBA:ARBA00025347}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by
CC hydrolysis of a carboxyamide bond of beta-aminoalanine, but also
CC shows general aminopeptidase activity. The specificity varies
CC somewhat with source, but amino acid arylamides of Met, Leu and Ala
CC are preferred.; EC=3.4.22.40;
CC Evidence={ECO:0000256|ARBA:ARBA00000423};
CC -!- SUBUNIT: Homohexamer. Binds to nucleic acids. Binds single-stranded DNA
CC and RNA with higher affinity than double-stranded DNA.
CC {ECO:0000256|ARBA:ARBA00026080}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAV20305.1}.
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DR EMBL; NBII01000003; PAV20305.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A286ULH3; -.
DR STRING; 2282107.A0A286ULH3; -.
DR InParanoid; A0A286ULH3; -.
DR OrthoDB; 45184at2759; -.
DR Proteomes; UP000217199; Chromosome 3.
DR GO; GO:0070005; F:cysteine-type aminopeptidase activity; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00585; Peptidase_C1B; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR004134; Peptidase_C1B.
DR PANTHER; PTHR10363; BLEOMYCIN HYDROLASE; 1.
DR PANTHER; PTHR10363:SF2; BLEOMYCIN HYDROLASE; 1.
DR Pfam; PF03051; Peptidase_C1_2; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:PAV20305.1};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000217199};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807}.
SQ SEQUENCE 544 AA; 60856 MW; 3C11F20C885EFACC CRC64;
MSLRDFFLVE DDLNCCCPQC ISCHIRPRNR TRARSNSCTG IRVTSMGAAQ STSAQSVSVA
SSSLSEKVPA QEYSVQPSLK AATPLSPNGS ISLKNVAQWE EAAVDDLKVQ LARTVLVDTD
YKSALATRSA RIADPHVFNV QLDFKTGPVT NQKSSGRCWL FATTNVLRHS IMQKLSLKEF
QLSQSYLFFW DKLNKCNYYL ELSIETADLA LDDRIVNFLS NDLISDGGQW DMAVNLLETY
GVVPQPIYPE SYSSSASSNL NKLLKLKLRE HALILRRLSA SLKGVTDKEQ ALSSIRAKKE
ELMSEIWTIM TATLGVPPRP DDAFTWEYLD GDGKFRSWTG TPLEFYKAFT SRQYPPTDSF
SIINDPRNEY KKLYTVDKLG NIWGGREVLY VNTHIDDLKN TIVKMLKAGQ PAFFGCDVNQ
FSDSRGGILD TGLHKAAIEN AFNITLGLTK AERLEMNESS MTHAMVITAA HVDASGRPVR
FRVENSWGPD SGEKGYLVMT DKWFDEFVYQ VVVPKSLAPK ELVKVFESRE KIVLPPWDPM
GSLA
//
