GenomeNet

Database: UniProt
Entry: A0A287ST51_HORVV
LinkDB: A0A287ST51_HORVV
Original site: A0A287ST51_HORVV 
ID   A0A287ST51_HORVV        Unreviewed;       740 AA.
AC   A0A287ST51;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   31-JUL-2019, entry version 12.
DE   SubName: Full=Urease {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.4};
OS   Hordeum vulgare subsp. vulgare (Domesticated barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=112509 {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.4, ECO:0000313|Proteomes:UP000011116};
RN   [1] {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.4, ECO:0000313|Proteomes:UP000011116}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=subsp. vulgare {ECO:0000313|Proteomes:UP000011116};
RX   PubMed=23075845; DOI=10.1038/nature11543;
RG   The International Barley Genome Sequencing Consortium;
RT   "A physical, genetic and functional sequence assembly of the barley
RT   genome.";
RL   Nature 491:711-716(2012).
RN   [2] {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.4}
RP   IDENTIFICATION.
RC   STRAIN=subsp. vulgare {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.4};
RG   EnsemblPlants;
RL   Submitted (OCT-2017) to UniProtKB.
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRSR:PIRSR611612-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRSR:PIRSR611612-51};
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR611612-50}.
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DR   EnsemblPlants; HORVU5Hr1G114090.4; HORVU5Hr1G114090.4; HORVU5Hr1G114090.
DR   Gramene; HORVU5Hr1G114090.4; HORVU5Hr1G114090.4; HORVU5Hr1G114090.
DR   OMA; GFDSHIH; -.
DR   Proteomes; UP000011116; Unassembled WGS sequence.
DR   ExpressionAtlas; A0A287ST51; baseline and differential.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:InterPro.
DR   GO; GO:0043419; P:urea catabolic process; IEA:InterPro.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000011116};
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR611612-51};
KW   Nickel {ECO:0000256|PIRSR:PIRSR611612-51};
KW   Reference proteome {ECO:0000313|Proteomes:UP000011116}.
FT   DOMAIN      311    740       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    502    502       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       316    316       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       318    318       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       399    399       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       399    399       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       428    428       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       454    454       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       542    542       Nickel 1. {ECO:0000256|PIRSR:PIRSR611612-
FT                                51}.
FT   BINDING     401    401       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     399    399       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
SQ   SEQUENCE   740 AA;  78916 MW;  EBE6C2F333DF2348 CRC64;
     MDGTKLITVH DPISSDDGNL ELALHGSYLP VPSLEKFSGS DVEDYPGEVH FCSGRIILNL
     HRRALTLKVV NKADRPIQIG SHYHFIEANP YLVFDRLRAY GMRLNIPAGT AVRFEPGDAK
     RVTLVSIGGR KVIRGGNGIA DGAVDSSQLN EVIQKITENC FGHEDYPDAS EGLIGDGIFD
     CSVDHEKYSS MYGPTTGDKI RLGDTDLFAE IEKDFAVYGD ECIFGGGKVL RDGMGQSAGY
     PASASLDTVI TNAVVIDYTG IYKADIGIKD GLIIAIGKAG NPDVMDGVHS NMIVGVNTEV
     IAAQGMIVTA GGIDCHVHFI CPQLINEAIA SGITTLVGGG TGPAHGTCAT TCTPAPSQMK
     LMLQSTDEFP INVGFTGKGN TAKPEGLSEI IKAGAMGLKL HEDWGSTPAA IDNCLSVAEA
     FDIQVNIHTD TLNEAGCVEH TIAAFKDRSI HTYHSEGAGG GHAPDIIKVC GVKNVLPSST
     NPTRPFTSNT VDEHLDMLMV CHHLDKNIPE DVAFAESRIR AETIAAEDIL HDMGAISIIS
     SDSQAMGRIG EVIIRTWQTA NKMKVQRGWL PGPGESDPAK YNDNFRIRRY IAKYTINPAI
     VSGFSDFVGS VEAGKLADLV LWKPAFFGAK PELIIKGGAI AWANMGDPNA SIPTPEPVMM
     RPMFGAYGKA GSSHSIAFVS KAAKEAGVAS EYKLAKRVEA VGGVRSLTKL DMKLNDALPK
     IEVDPETYTV TADGEVLTCQ
//
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