ID A0A291ISF8_9MOLU Unreviewed; 446 AA.
AC A0A291ISF8;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN Name=dnaB {ECO:0000313|EMBL:ATG97674.1};
GN Synonyms=dnaC {ECO:0000313|EMBL:MCL8216724.1};
GN ORFNames=CP520_02960 {ECO:0000313|EMBL:ATG97674.1}, LD118_00306
GN {ECO:0000313|EMBL:MCL8216724.1};
OS Mesoplasma lactucae ATCC 49193.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales;
OC Entomoplasmataceae; Mesoplasma.
OX NCBI_TaxID=81460 {ECO:0000313|EMBL:ATG97674.1, ECO:0000313|Proteomes:UP000232227};
RN [1] {ECO:0000313|EMBL:ATG97674.1, ECO:0000313|Proteomes:UP000232227}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=831-C4 {ECO:0000313|EMBL:ATG97674.1,
RC ECO:0000313|Proteomes:UP000232227};
RA Knight T.F., Rubinstein R., Citino T.;
RT "SPAdes assembly of the Mesoplasma lactucae genome.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MCL8216724.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 49193 {ECO:0000313|EMBL:MCL8216724.1};
RA Green E.A., Klassen J.;
RT "Mollicute symbiont genomes associated with the fungus-growing ant,
RT Trachymyrmex septentrionalis.";
RL Submitted (FEB-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
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DR EMBL; CP023668; ATG97674.1; -; Genomic_DNA.
DR EMBL; JAKNSY010000001; MCL8216724.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A291ISF8; -.
DR KEGG; mlac:CP520_02960; -.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000232227; Chromosome.
DR Proteomes; UP001139645; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR NCBIfam; TIGR00665; DnaB; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS51199; SF4_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:ATG97674.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085}; Primosome {ECO:0000256|RuleBase:RU362085};
KW Reference proteome {ECO:0000313|Proteomes:UP000232227}.
FT DOMAIN 176..446
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 179..363
FT /note="RecA family profile 1"
FT /evidence="ECO:0000259|PROSITE:PS50162"
SQ SEQUENCE 446 AA; 50624 MW; 073A3807ADFB2875 CRC64;
MNNEQQSQIL EEAEQQVLAT AIKSPNALPD ILISLTHDDF FFGQHNLIFQ AIENLNSNNQ
EITELTIASW LDKNGKLQQA GGNAKIFEIS SIYYTDENIE SYIDIIHEAS AERKFKNLIR
KTSEETNKTS VNYRKLLEKA QTDLLEIDFD IKKNDTTKIG DSAINVVKRI KQLAESKESL
TGVSTGFHEL DNVTSGLQPQ DFIILAARPS MGKTAFALNL AYNAATDRQQ DNAVAIFSVE
MPTDQLTQRI LSMETQINAD KLRNGKGITK EEWRKINAAE DKLRKTRIFV DDTPGITVQQ
IQSKLHKLKR DENVNLCIID YLQLISTPGS NGNDRQNEIS TISRQLKRIA RDTGVTIVCL
SQLSRSVEKR EDKRPIMSDL RDSGAIEQDA DMIMFLYRKD YYQHNDDEED TSEVSPTELI
IAKHRNGATK DISLNFLKQY GKFLDK
//
