ID A0A291JHZ1_9STAP Unreviewed; 476 AA.
AC A0A291JHZ1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=protein-N(pi)-phosphohistidine--sucrose phosphotransferase {ECO:0000256|ARBA:ARBA00044053};
DE EC=2.7.1.211 {ECO:0000256|ARBA:ARBA00044053};
GN ORFNames=BJD96_02900 {ECO:0000313|EMBL:ATH59362.1};
OS Staphylococcus nepalensis.
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=214473 {ECO:0000313|EMBL:ATH59362.1, ECO:0000313|Proteomes:UP000217956};
RN [1] {ECO:0000313|EMBL:ATH59362.1, ECO:0000313|Proteomes:UP000217956}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JS1 {ECO:0000313|EMBL:ATH59362.1,
RC ECO:0000313|Proteomes:UP000217956};
RA Nam Y.-D., Kang J., Chung W.-H.;
RT "Complete genome sequence of Staphylococcus nepalensis JS1.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(pros)-phospho-L-histidyl-[protein](out) + sucrose = L-
CC histidyl-[protein] + sucrose 6(G)-phosphate(in);
CC Xref=Rhea:RHEA:49236, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:17992, ChEBI:CHEBI:29979, ChEBI:CHEBI:64837,
CC ChEBI:CHEBI:91002; EC=2.7.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00043763};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CP017460; ATH59362.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A291JHZ1; -.
DR KEGG; snl:BJD96_02900; -.
DR Proteomes; UP000217956; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00212; PTS_IIB_glc; 1.
DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1.
DR InterPro; IPR036878; Glu_permease_IIB.
DR InterPro; IPR018113; PTrfase_EIIB_Cys.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR001996; PTS_IIB_1.
DR InterPro; IPR010973; PTS_IIBC_sucr.
DR NCBIfam; TIGR00826; EIIB_glc; 1.
DR NCBIfam; TIGR01996; PTS-II-BC-sucr; 1.
DR PANTHER; PTHR30175; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1.
DR PANTHER; PTHR30175:SF4; PTS SYSTEM TREHALOSE-SPECIFIC EIIBC COMPONENT; 1.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF55604; Glucose permease domain IIB; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 112..139
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 186..205
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 264..282
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 303..326
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 346..369
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 402..420
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 4..87
FT /note="PTS EIIB type-1"
FT /evidence="ECO:0000259|PROSITE:PS51098"
FT DOMAIN 120..476
FT /note="PTS EIIC type-1"
FT /evidence="ECO:0000259|PROSITE:PS51103"
FT ACT_SITE 26
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421"
SQ SEQUENCE 476 AA; 50927 MW; BBABD5EC03C9640B CRC64;
MNYKKSAESI LNAIGGENNI DAMAHCATRL RLVLKDESLI DEESLSSMEV VKGTFATGGQ
YQIIIGSGTV NKVFAELEKI TGKEASATSE VKSEGNKSMN PFQKFVKMLS DIFVPIIPAI
VAGGLLMGIN NILTAPGIFY NHHSLIEVHS QFKGLAEMID IFANAPFTLL PILIGFSAAK
RFGGNAFLGA ALGMILVHPE LMSAYDYPKA VELGKSIPHW HLFGLEINRV GYQGQVLPML
VATYILAKLE QGLRKVVPTV LDNLLTPLIS ILVTAFITFL FVGPITRTLG YWLSDGLTWL
YEFGGAIGGL IFGLLYAPIV ITGMHHSFIA IETQLIADNA TTGGSFIFPI ATMSNVAQGA
AALAAFLIIK KNKKLKGIAS AAGISALLGV TEPAMFGVNL KLRYPFIGAI IGSGIGAAYI
SFFKVKAIAL GTAGLPGFIS ISGVNHGWLH YGIGIAITFI VTFVVTYALS FRKKYV
//
