ID A0A2A2E0N1_9PSED Unreviewed; 853 AA.
AC A0A2A2E0N1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Nitrite reductase large subunit {ECO:0000313|EMBL:PAU61869.1};
GN ORFNames=BZL43_03905 {ECO:0000313|EMBL:PAU61869.1};
OS Pseudomonas sp. PICF141.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1949067 {ECO:0000313|EMBL:PAU61869.1, ECO:0000313|Proteomes:UP000217819};
RN [1] {ECO:0000313|EMBL:PAU61869.1, ECO:0000313|Proteomes:UP000217819}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PICF141 {ECO:0000313|EMBL:PAU61869.1,
RC ECO:0000313|Proteomes:UP000217819};
RA Gomez-Lama C., Legarda-Cristobal G., Trivino J.C., Mercado-Blanco J.;
RT "Genome sequences of Pseudomonas strains PIC25, PIC105 and PICF141
RT indigenous of the olive (Olea europaea L.) cv. Picual rhizosphere and
RT effective biological control agents against Verticillium dahliae.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR037149};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAU61869.1}.
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DR EMBL; MUNM01000024; PAU61869.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A2E0N1; -.
DR OrthoDB; 9768666at2; -.
DR UniPathway; UPA00653; -.
DR Proteomes; UP000217819; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniRule.
DR CDD; cd19944; NirB_Fer2_BFD-like_2; 1.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR012744; Nitri_red_NirB.
DR InterPro; IPR017121; Nitrite_Rdtase_lsu.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR NCBIfam; TIGR02374; nitri_red_nirB; 1.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PIRSF; PIRSF037149; NirB; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRNR:PIRNR037149};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|PIRNR:PIRNR037149}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063,
KW ECO:0000256|PIRNR:PIRNR037149};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 14..293
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 327..397
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 428..476
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 566..628
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 638..759
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
SQ SEQUENCE 853 AA; 92632 MW; 7C8586F3C22FE2A7 CRC64;
MNSNVAALNK LQTLIVIGNG MVGHHCVEQL IERGALDHYR LHVFSEEPMR AYDRVHLSEY
FGGRDAESLA LGEASLYQTP GVTLHLGVPV LEIDRDRRQV ITAQGCVAYD KLVLATGSYP
FVPPIEGAEG DSRLVYRTLE DLDAIRAAAA NARRGVVVGG GLLGLEAANA LKSLGLEAHV
VEFAPRLMPV QLDEQGGLAL KAQIERLGVS VHLSRGTQSI TAGEQYRYRM NFANDEFLEA
DLIVFSAGIR AQDALARQCA LDIGPRGGVV IDDQCLTSDP NIYAIGECAA WNGSLFGLVA
PGYQMARGVA TLLCNQVAEP FQGADMSTKL KLLGVDVGSI GDAHAQTPGA RSYQFIDEAT
ASYRRLVVDA DSKRVLGAVL VGDNSYYDTL LQYMQNGIAL PSEPASLILP SSTGAPTLGP
GALPEAATVC SCHNVTKGSI CSAIDGGCTD LGLLKSQTKA CTGCGGCAGL LKQVFEHELI
ARGVSVDKSL CEHFAYTRQE LYALVRVEGV ITFEELLAKH GRGHTGCDVC KPAVGSILAS
CWNQPIMDAS LVPLQDTNDT FMANMQKNGT YSVVPRIAGG EITADKLIAI GVVAKKYDLY
TKITGGQRID LFGAQLHQLP DIWSELIEAG FETGHAYGKS TRTVKSCVGS TWCRYGVQDS
VKMALTLEDR YKGLRSPHKL KFAVSGCTRE CAEAQSKDVG VIATENGWNL YVAGNGGMRP
RHAELFATDL DDETLIRYID RFLMFYIRTA DKLQRTSVWR ESLEGGLDYL KDVIINDSLG
LGAELESQMQ LVVNRYECEW ANALKDPEKL KRFRTFVNDQ RPDPDIHFVQ ERGQRRPIMA
AELNLIPVTE ENA
//
