ID A0A2A2EDG3_9BIFI Unreviewed; 1183 AA.
AC A0A2A2EDG3;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=B1526_1349 {ECO:0000313|EMBL:PAU67264.1};
OS Bifidobacterium criceti.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=1960969 {ECO:0000313|EMBL:PAU67264.1, ECO:0000313|Proteomes:UP000218399};
RN [1] {ECO:0000313|EMBL:PAU67264.1, ECO:0000313|Proteomes:UP000218399}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=71 {ECO:0000313|EMBL:PAU67264.1,
RC ECO:0000313|Proteomes:UP000218399};
RX PubMed=28837128; DOI=10.1038/ismej.2017.138;
RA Milani C., Mangifesta M., Mancabelli L., Lugli G.A., James K., Duranti S.,
RA Turroni F., Ferrario C., Ossiprandi M.C., van Sinderen D., Ventura M.;
RT "Unveiling bifidobacterial biogeography across the mammalian branch of the
RT tree of life.";
RL ISME J. 11:2834-2847(2017).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAU67264.1}.
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DR EMBL; MVOH01000015; PAU67264.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A2EDG3; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000218399; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 648..809
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 834..984
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1183 AA; 131082 MW; F6A9DF8EF70298CB CRC64;
MPADMNEHEL HHGSLAALRA ALDADPTFHD LAHGLADTPD AAGDPSLVIG APDGIRPALA
AARAALDPIV IVVPSGREAE ELVDDVRSWY DGDPAAVAQL EAWETLPHER LSPRADTVAN
RMEVFRRLCH PEPGNAMFGP IRILVMPVRS LIQPVVAGIG DVEPLVFTVG EEVALDEASA
ALVRNAYTRV DLVMDRGEFA VRGGLIDVFP PTLPHPVRIE FFGDEIESIR EFHASDQRTY
GGTIETVWAT PCRELQLTPE VRERARSLIG RIPNADDMLE SIAQAIPVEG MESLMPALID
DMCPVSSMLP RRSVVMLCDP EKLRRAVDDL AKTANEFLAT SWHVAASGHG AGAPITFDGA
NFLAYDEVVS SLAYSERELW KVSGFTVDTS MPGHLQIDAQ TPREFRGDER RTEQGVRTLL
ADGYAVTLTA AAQGTLHRLT RMLNAVGVTD VTPVRSQARD GFIEHRAKVA LLTERDITGR
ESAAGGPKTP RKRRKSIDLM ELKPGDFVVH EQHGIGRFIE MRQRTTGSGR NRTTREYLVI
EYAPGKRNAP PDKLFIPTDQ LDLVSKYIGA EIPKLNKLGG SDWAATKAKA RKHVHEIAQN
LIKLYSARQR TKGFAFSKDT PWQKELEDAF PYQETADQLT TIDDVKADME KPLPMDRLIC
GDVGFGKTEI AVRAAFKAVQ DSKQVAVLVP TTLLVQQHYE TFTERFEGFP VNVAAMSRFQ
STKQINETIK GLADGSVDVV IGTHKLLNPK ITFKDLGLVI IDEEQRFGVE AKEALKAIRT
NVDVLSLSAT PIPRTLEMAV TGIREMSTLA TPPEDRLPVL TYVGAYEDAQ VTAAIRRELL
RGGQVFYIHN RVEDIDKKAA KLHELVPEAR IGIAHGKMGR KQLDQVIRDF WHRDIDVLLC
TTIVETGLDI SNANTLIVDH ADRFGLSQLH QLRGRVGRGR ERAYAYFLYD PGKPMTEQSH
ERLVTIAQNT ALGSGFDVAM KDLELRGTGN LLGDEQSGHI EGVGFDLYVR MVSEAIEDYK
EPDRTEPPVV SIDLPIEASI PVDYIDADKL RLEAYRKLAE ARDDDDLEEL REELTDRYGK
PPQVFTSLFD IARLRERARG LGITEIVGQG GKLRVGGIDP PESVRMRIER IYKGAQYRPL
THTYMIPTPF EGTLGSGPMS SDDVIKWTEQ LLDDLAWTPK PRT
//
