ID A0A2A2GTG4_9HELI Unreviewed; 905 AA.
AC A0A2A2GTG4;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=B9T66_02210 {ECO:0000313|EMBL:PAV00482.1};
OS Helicobacter sp. TUL.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1848928 {ECO:0000313|EMBL:PAV00482.1, ECO:0000313|Proteomes:UP000218502};
RN [1] {ECO:0000313|EMBL:PAV00482.1, ECO:0000313|Proteomes:UP000218502}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TUL {ECO:0000313|EMBL:PAV00482.1,
RC ECO:0000313|Proteomes:UP000218502};
RA Van Der Mee-Marquet N., Benejat L., Diene S.M., Gaia N., Cherkaoui A.,
RA Lemaignen A., Ducournau A., Lacomme S., Gontier E., Bernard L., Megraud F.,
RA Goudeau A., Lehours P., Francois P.;
RT "Helicobacter caesarodunum: a novel human pathogen identified in a
RT bloodstream infection.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAV00482.1}.
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DR EMBL; NESU01000003; PAV00482.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A2GTG4; -.
DR Proteomes; UP000218502; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000218502};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 1..260
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 317..498
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 669..869
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 905 AA; 103374 MW; A495BC92EEF2A6AB CRC64;
MKTLTIIDTF GFFFRSFYAL PPLKNKEGFP TGLLLGFCNL LNNLYKDKNC DYILFALEGQ
GKNRRKILDP RYKQNRQEVP KELLLQLPIA IEWIQKMGFV NLSVDGYEAD DVIASVSVLA
HKKGLNVRII SHDKDLYQLI DSQTYIYDPI KKIEIHEEQC QEKFGVLPCE FIDYQSLVGD
SSDNVAGVKG IGAKSAQKLI KHFQNLESLY QRSDEIAQVL TPRLQKIILD SKDEAFLAKS
LVTLVKDIPL EIDFAQCAMP SQNPLLTIVD ALHKYEFNGI LTKIQGPKYN SLSTAPSFKN
ATLSQQISQS STFTYEYEMI DSQSTLFALI QSIPKDALIA FDTESDGLDT KEANIVGFSF
SIDGHKGYYV PLGHTYLGVQ TQIDKQSAKV ALESIFSHTL IGHNLKFDIA LVYHNFGIKP
QNTIIDTMVL AWLLDSAKPV GLDKQMLQWF GYKMISFEEI VDKKQTFANV DINLASQYAA
EDAVATYCLY KRLKQEFEQR GLEPILELAR ELEFPFINVL VQMEMEGIKI DTTLFESYNK
DFTKILHSLT QQIYECAGEN FNINSPQQLS QILFTKLGLQ SGRSVKGGLS TDEHTLSNIA
HSHPIIPLVL DYREMNKLKN TYIEPLLKYA NKNAEHKIYT SFLQTGTVTG RLSSKSPNLQ
NIPVRSEYGR KIRKGFIAKE GHKLISVDYS QIELRLLAHF SQDSHLIEAF KQQKDIHYET
ALRLFGEDQA HNKRFIAKTI NFGLIYGMGA RKLSETLKIT HKEAKEYIES YFKLFPTVKD
YLHNQKEFLL ENGYTQTLLG RRRYFDFSDA TEFMKANYIR EGINCIFQGS AADLIKLCMY
KIYQHYQNTE LQMLLQVHDE LIFQAPESKV GEFLPHIESL MNSIYTLLVP LKCSVSVGDN
WADLK
//
