ID A0A2A2JB25_9BILA Unreviewed; 385 AA.
AC A0A2A2JB25;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Acid ceramidase {ECO:0000256|ARBA:ARBA00040588};
DE EC=3.5.1.23 {ECO:0000256|ARBA:ARBA00011891};
GN ORFNames=WR25_10479 {ECO:0000313|EMBL:PAV58936.1};
OS Diploscapter pachys.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Diploscapter.
OX NCBI_TaxID=2018661 {ECO:0000313|EMBL:PAV58936.1, ECO:0000313|Proteomes:UP000218231};
RN [1] {ECO:0000313|EMBL:PAV58936.1, ECO:0000313|Proteomes:UP000218231}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PF1309 {ECO:0000313|EMBL:PAV58936.1};
RA Fradin H., Zegar C., Gutwein M., Lucas J., Kovtun M., Corcoran D.,
RA Baugh L.R., Kiontke K., Gunsalus K., Fitch D.H., Piano F.;
RT "Genome architecture and evolution of a unichromosomal asexual nematode.";
RL Curr. Biol. 0:0-0(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine;
CC Xref=Rhea:RHEA:20856, ChEBI:CHEBI:15377, ChEBI:CHEBI:28868,
CC ChEBI:CHEBI:52639, ChEBI:CHEBI:57756; EC=3.5.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00036977};
CC -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC {ECO:0000256|ARBA:ARBA00004760}.
CC -!- PATHWAY: Sphingolipid metabolism. {ECO:0000256|ARBA:ARBA00004991}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the acid ceramidase family.
CC {ECO:0000256|ARBA:ARBA00005730, ECO:0000256|PIRNR:PIRNR017632}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAV58936.1}.
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DR EMBL; LIAE01010554; PAV58936.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A2JB25; -.
DR STRING; 2018661.A0A2A2JB25; -.
DR Proteomes; UP000218231; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0017064; F:fatty acid amide hydrolase activity; IEA:InterPro.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:InterPro.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:UniProtKB-KW.
DR CDD; cd01903; Ntn_AC_NAAA; 1.
DR InterPro; IPR016699; Acid_ceramidase-like.
DR InterPro; IPR029130; Acid_ceramidase_N.
DR InterPro; IPR029132; CBAH/NAAA_C.
DR PANTHER; PTHR28583; ACID AMIDASE; 1.
DR PANTHER; PTHR28583:SF1; ACID CERAMIDASE; 1.
DR Pfam; PF02275; CBAH; 1.
DR Pfam; PF15508; NAAA-beta; 1.
DR PIRSF; PIRSF017632; Acid_ceramidase-like; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR017632};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW ECO:0000256|PIRNR:PIRNR017632};
KW Reference proteome {ECO:0000313|Proteomes:UP000218231};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Sphingolipid metabolism {ECO:0000256|ARBA:ARBA00022919}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..385
FT /note="Acid ceramidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5013330862"
FT DOMAIN 47..84
FT /note="Acid ceramidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF15508"
FT DOMAIN 131..277
FT /note="Choloylglycine hydrolase/NAAA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02275"
FT ACT_SITE 131
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017632-1"
SQ SEQUENCE 385 AA; 43658 MW; B9F503CE8DCC5A5A CRC64;
MRCLTLLALL VTIFVVSPKH VDLPKPFADD CILNDGKNVY DPSRQFEIPW FDVNLDAAPK
DRWTHIAASL INFVTPIFPG ALEFVDHTFG DMGSKLPMPY RDEIQGISDA TGIPLGRIVV
YNIFYEIFTV CTSIVAQDSQ GHLVHARNLD FGLFLGWDMN SHEWTVSEKL RKMIINVNWI
KNGKLLFKSN EFAGYIGIYN GMKPNAFSLT CDDRFQKAGG YYGIIKWLLG LEPESRWMSW
LSRETLENCN SYAEAKEHLM NTPMLSPVYF ILGGVNPKEG AIIARSLNST ALLTEMGVNS
ANDWYVLETN YDQDTEPLYL DDRDTPGNNC MHKLTKDGVS FQGIFNVLSS RTNLNKLTTY
TVLMSTYDSK FETILQSCPG ECWPW
//
