ID   A0A2A2K0A2_9BILA        Unreviewed;       539 AA.
AC   A0A2A2K0A2;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 13.
DE   RecName: Full=Amidase domain-containing protein {ECO:0000259|Pfam:PF01425};
GN   ORFNames=WR25_15193 {ECO:0000313|EMBL:PAV67312.1};
OS   Diploscapter pachys.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Diploscapter.
OX   NCBI_TaxID=2018661 {ECO:0000313|EMBL:PAV67312.1, ECO:0000313|Proteomes:UP000218231};
RN   [1] {ECO:0000313|EMBL:PAV67312.1, ECO:0000313|Proteomes:UP000218231}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PF1309 {ECO:0000313|EMBL:PAV67312.1};
RA   Fradin H., Zegar C., Gutwein M., Lucas J., Kovtun M., Corcoran D.,
RA   Baugh L.R., Kiontke K., Gunsalus K., Fitch D.H., Piano F.;
RT   "Genome architecture and evolution of a unichromosomal asexual nematode.";
RL   Curr. Biol. 0:0-0(2017).
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PAV67312.1}.
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DR   EMBL; LIAE01009955; PAV67312.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A2K0A2; -.
DR   STRING; 2018661.A0A2A2K0A2; -.
DR   Proteomes; UP000218231; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1300.10; Amidase signature (AS) domain; 1.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR45847; FATTY ACID AMIDE HYDROLASE; 1.
DR   PANTHER; PTHR45847:SF1; MONOGLYCERIDE LIPASE FAAH-4; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   PIRSF; PIRSF001221; Amidase_fungi; 2.
DR   SUPFAM; SSF75304; Amidase signature (AS) enzymes; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000218231};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        17..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          103..491
FT                   /note="Amidase"
FT                   /evidence="ECO:0000259|Pfam:PF01425"
FT   ACT_SITE        157
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        232
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT   ACT_SITE        256
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
SQ   SEQUENCE   539 AA;  60583 MW;  74CFDA4531DD1829 CRC64;
     MGNALVSELE TWTVKQWLWT AGFGTFVIFV VSKFIFAKPW GIIRNYKRAI EQNNERRRKG
     FAEFEERIRT EDAQVNDKRK KILGLSLLQL REALQKDHFT AMEVLEAYVE KAMQIQKEIN
     CCTQVISEAF DYAREADEKW SGKSDKPPLY GLPFSVKENF HLKGYDCTVG VAKFLNQPKS
     HDCSLVVHLK QLGAVPFVVT NVPQGLISFV CSNTVYGTTS NPHDHSRTPG GSSGGEAALY
     ASGGSAFGIG SDLAGSLRIP ASLCGVVSFK PTQDRFIVKR SHDGIPGRGR LGLSFGFFTS
     SVDEQMFLLE CCIGTDAYRQ IVPQTPPAPL RLEMIDASKK MKFGYFETDG FADPVPAMRR
     SVIETVEKLK SEGHELVKFE VPDVRNMIDI MYKNILPDQG AYMKFIYGND LVDENLKIFV
     FLLNVPRSIR KLTSYLVAGI SPQLSAICAA SVRDLSDLRY TQELTDNYRQ KFIDYWKSLG
     IDALICPAFI GYNMLLKDQR EAAKNSEGLP CSVQIVTLPY EEELCLHAMK QVEAVWRKN
//