ID A0A2A3AH68_9MICC Unreviewed; 337 AA.
AC A0A2A3AH68;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=CKW39_04600 {ECO:0000313|EMBL:PBB08862.1};
OS Kocuria sp. WRN011.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Kocuria.
OX NCBI_TaxID=2029858 {ECO:0000313|EMBL:PBB08862.1, ECO:0000313|Proteomes:UP000218573};
RN [1] {ECO:0000313|EMBL:PBB08862.1, ECO:0000313|Proteomes:UP000218573}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WRN011 {ECO:0000313|EMBL:PBB08862.1,
RC ECO:0000313|Proteomes:UP000218573};
RA Zhang G., Wu G.;
RT "Kocuria alkalisoli sp. nov., isolated from saline alkaline soil.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PBB08862.1}.
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DR EMBL; NSGI01000003; PBB08862.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A3AH68; -.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000218573; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR CDD; cd04730; NPD_like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001295; Dihydroorotate_DH_CS.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
DR PROSITE; PS00912; DHODEHASE_2; 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Dioxygenase {ECO:0000313|EMBL:PBB08862.1};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000218573}.
FT DOMAIN 9..333
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 337 AA; 34866 MW; 3B3FA682C3E61CB9 CRC64;
MTTMLDTLCV PLIQAPMAGG PSNAALAVAV SQAGGLGMLA AGYKTVEAMA AEIDDVRAGT
LNFGVNLFVP EADPSDPEAL DAYATALAPM AAELGIEAPE PGPFNDDQYP AKVDYLVANP
VPLVSFTFGL PTADDVARLR SVGTEIVLNA TDPEEVSAAD ALHPDAIVVQ GAEAGGHRAT
HGQAKEPSES STHELVSLAR EYTDRHVIAA GGVTSPEIAQ NLLGAGASAV QVGTLFLTAD
EAGTKDAHKD ALVNGQHRDT VVTRAFSGRA ARALSNRFTE RMAANQVVGY PQVHYMTAPL
RAASADNPEG LNLWAGTGFA VCQARPAAEI VAEFRSL
//
