ID   A0A2A3I1H6_9ACTN        Unreviewed;       816 AA.
AC   A0A2A3I1H6;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   ORFNames=BX265_2187 {ECO:0000313|EMBL:PBC77439.1};
OS   Streptomyces sp. TLI_235.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1938860 {ECO:0000313|EMBL:PBC77439.1, ECO:0000313|Proteomes:UP000218662};
RN   [1] {ECO:0000313|EMBL:PBC77439.1, ECO:0000313|Proteomes:UP000218662}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TLI_235 {ECO:0000313|EMBL:PBC77439.1,
RC   ECO:0000313|Proteomes:UP000218662};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PBC77439.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; NSGV01000001; PBC77439.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A3I1H6; -.
DR   OrthoDB; 9806486at2; -.
DR   Proteomes; UP000218662; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 2.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:PBC77439.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000218662};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          21..476
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
FT   REGION          784..816
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   816 AA;  87660 MW;  C6328820248C1C87 CRC64;
     MARRSSPTPP PGDFEERILD VDVVDEMQGS FLEYAYSVIY SRALPDARDG LKPVHRRILY
     QANEMGLRPE RGHVKCARLV GEVMGRLHPH GDASIYDSVV RMAQPFSMRL PLIDGHGNFG
     SLGNDDPPAA MRYTESRLTA ASMSMVESIH EDTVDFGPNY DGSEQEPQVL PSAFPNLLVN
     GASGIAVGMA TNMPPHNLAE VVAAARHLIK HPNADLDTLM RFVPGPDLPT GGRIVGLSGI
     RDAYANGRGT FKIRATTTIE SVSARRKGIV VTELPYSVGP EKVIAKIKDL VNAKKLQGIA
     DVKDLTDREN GLRLVIEVKN GFVPEALLEQ LFKLTPMEET FGINNVALVD GQPLTLGLKE
     LLEVYVDHRF EVVRRRSDFR RRKRQERLHL VEGLLVALVD IDEVIALIRS SDNAAQAKER
     LIERFSLSEV QTAYILDTPL RRLTRFDRVE LEDEQRKLTA EIAELTEILE SDSRLRSVVS
     SELGAVSKQF GTARRTVLLE AGAVPSAALS VPLEVADDPC RVLLSSTGLL VRTPDAASVA
     VPEQRAKHDV VVSAVAATAR ADVGVVTSAG RVLRLPVIDL PQLPPGGASS LAGGAAVSEF
     LRLEAGERAL ALTTLDESSP GLALGTVQGV VKRVVPEWPA NKDEFEVIAL REGDEVVGAV
     ELATGEEDLV FITSDAQLLR YPAGQVRPQG RPAGGMAGVK VSDGARVLSF TAVDPAADAV
     VLSVAAASGT LSGGEQTTWK MTPFELYPRK GRATGGVRCQ RFLRGEDVLA FAWAGAAPAL
     AAAANGSPVE LPERDPRRDG SGSPVTAQVA TVGGRM
//