ID   A0A2A3XJ04_9GAMM        Unreviewed;      1168 AA.
AC   A0A2A3XJ04;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=CIK78_17420 {ECO:0000313|EMBL:PCC23691.1};
OS   Halomonas sp. JB37.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Halomonas.
OX   NCBI_TaxID=2024405 {ECO:0000313|EMBL:PCC23691.1, ECO:0000313|Proteomes:UP000217454};
RN   [1] {ECO:0000313|EMBL:PCC23691.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JB37 {ECO:0000313|EMBL:PCC23691.1};
RX   PubMed=28644126;
RA   Bonham K.S., Wolfe B.E., Dutton R.J.;
RT   "Extensive horizontal gene transfer in cheese-associated bacteria.";
RL   Elife 6:e22144-e22144(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PCC23691.1}.
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DR   EMBL; NRGW01000001; PCC23691.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A3XJ04; -.
DR   OrthoDB; 9803237at2; -.
DR   Proteomes; UP000217454; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR048472; DNA_pol_IIIA_C.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          6..73
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
SQ   SEQUENCE   1168 AA;  130831 MW;  FDC153F3A74A6AF3 CRC64;
     MTVPFVHLRL HSEYSLVDGL VKLKSLVSTT AERAMPALAL TDETNLFGLV KFYKAAQGAG
     LKPLIGSDLW LQNPHDESHP YRITLLAMND VGYRNLTELI SKGWTHGQRQ GRAILDRQWV
     MEQSEGLIAL SGAREGEIGR HLLSDHEQDA RALLKEWQAA FPERFYLELV RTGRPLEEAC
     VHASVKLAIE TGTPVVATND VRFLAREDYW AHETRVAIGE GKALDDPRRE RRYTEEQYLK
     SPDEMAALFS DIPEALENSV MIAQRCSVNV RLGEIFLPEF EIPEGMTQDE FFRKVSHDGL
     TDRLDFLFPV ERYPRESDEY KAIEQRYRER LEFELNVIIQ MGFPGYFLIV MDFIQWAKDN
     SVPVGPGRGS GAGSLVAYAQ KITDLDPIGY DLLFERFLNP ERVSMPDFDV DFCMEKRDKV
     IEYVAERYGR NAVSQIVTFG TMAAKAVVRD VARAQGRPYS LGDKLSKLIP FEVGMTLSKA
     IEQEPALKEF IGNDEEAEEI WEMALKLEGT TRGTGKHAGG VVIAPTKLTD FSPLLCDEDG
     SGLVVQFDKN DIEEAGLVKF DFLGLRTLTI IDWALEMVDK VRSVNGQGAL NIDSIPLDDA
     PTFEMLKRAE TTAVFQLESR GMKELIKRLL PDSLDDMIAL VALFRPGPLQ SGMVDDFINR
     KHGRAEVSYP HPDYQHELLK PVLAPTYGII LYQEQVMQIA QVMAGYSLGQ ADMLRRAMGK
     KKPEEMAKQR DGFMDGCAAN GIDKNLAGNI FDLVEKFAGY GFNKSHSAAY ALVSYQTAWL
     KAHYPGPFMA AVMSTEMDNL DKVVPLIEEC RNLKLTVTPP DVNVGGYKFT VDTDARVVYG
     LGAIRGVGEG PIGAIVDARK ADGPFKDIFD FCRRMDPKRM NKRTLEALIR SGALDTIGPN
     RAVLFAAMED ALKAAAQNHT NQNLGMLDMF GDAFAEADDS ESNVYTEYLS AREWTDRERL
     SGEKDTLGLY LTGHPIDEYE RELKRFVSTR ISDLKPSRDP QRVAGLVVGV RTMKSKRGDT
     MAFITLDDRT GRIEASLFGE LFEQLRGQIE SDQVLIVEGE VSSDEFSGGL RLRGKDVTPM
     VTARIRYGQA VELALDAEQI NGRLIESLRD SLTPYRDEAG LPVRLQYRHP AAVAWLELGD
     EWKVAPSDDL LLALRDVQGQ AGVQLRYR
//