ID   A0A2A3YKT4_9MICO        Unreviewed;       193 AA.
AC   A0A2A3YKT4;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=HIT family hydrolase {ECO:0000313|EMBL:PCC39924.1};
GN   ORFNames=CIK66_06740 {ECO:0000313|EMBL:PCC39924.1};
OS   Brachybacterium alimentarium.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermabacteraceae;
OC   Brachybacterium.
OX   NCBI_TaxID=47845 {ECO:0000313|EMBL:PCC39924.1, ECO:0000313|Proteomes:UP000218598};
RN   [1] {ECO:0000313|EMBL:PCC39924.1, ECO:0000313|Proteomes:UP000218598}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=341_9 {ECO:0000313|EMBL:PCC39924.1,
RC   ECO:0000313|Proteomes:UP000218598};
RX   PubMed=28644126;
RA   Bonham K.S., Wolfe B.E., Dutton R.J.;
RT   "Extensive horizontal gene transfer in cheese-associated bacteria.";
RL   Elife 6:e22144-e22144(2017).
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PCC39924.1}.
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DR   EMBL; NRGR01000011; PCC39924.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A3YKT4; -.
DR   OrthoDB; 9784774at2; -.
DR   Proteomes; UP000218598; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   CDD; cd01275; FHIT; 1.
DR   Gene3D; 3.30.428.10; HIT-like; 1.
DR   InterPro; IPR039383; FHIT.
DR   InterPro; IPR011146; HIT-like.
DR   InterPro; IPR036265; HIT-like_sf.
DR   PANTHER; PTHR42997:SF1; AP-4-A PHOSPHORYLASE; 1.
DR   PANTHER; PTHR42997; HIT FAMILY HYDROLASE; 1.
DR   Pfam; PF01230; HIT; 1.
DR   SUPFAM; SSF54197; HIT-like; 1.
DR   PROSITE; PS51084; HIT_2; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000313|EMBL:PCC39924.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000218598}.
FT   DOMAIN          50..159
FT                   /note="HIT"
FT                   /evidence="ECO:0000259|PROSITE:PS51084"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           144..148
FT                   /note="Histidine triad motif"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00464"
FT   ACT_SITE        146
FT                   /note="Tele-AMP-histidine intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-1"
FT   BINDING         76
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
FT   BINDING         148
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639383-2"
SQ   SEQUENCE   193 AA;  21030 MW;  03A2699AD3CF0AB4 CRC64;
     MDGSSPPTVE DARSFAGVPD GTERLWTPHR MAYIGGENKP SDPDDGEQCP FCSAQHRTDE
     DALIVHRGRV AYVILNLYPY NAGHLLVCPY RHVADYTELT DDEVREVAEL TRTAMRVIRQ
     VSAPAGFNLG MNQGPVAGAG IAAHLHQHVV PRWMGDANFL PIVGRTKAVP ILLADTRRLY
     AEAWPEGDGS GGS
//