ID A0A2A4J898_HELVI Unreviewed; 1277 AA.
AC A0A2A4J898;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=B5V51_5504 {ECO:0000313|EMBL:PCG68191.1};
OS Heliothis virescens (Tobacco budworm moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC Noctuidae; Heliothinae; Heliothis.
OX NCBI_TaxID=7102 {ECO:0000313|EMBL:PCG68191.1, ECO:0000313|Proteomes:UP000218220};
RN [1] {ECO:0000313|EMBL:PCG68191.1, ECO:0000313|Proteomes:UP000218220}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HvINT- {ECO:0000313|EMBL:PCG68191.1};
RC TISSUE=Whole body {ECO:0000313|EMBL:PCG68191.1};
RA Fritz M.L., Deyonke A.M., Papanicolaou A., Micinski S., Westbrook J.,
RA Gould F.;
RT "Contemporary evolution of a Lepidopteran species, Heliothis virescens, in
RT response to modern agricultural practices.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PCG68191.1}.
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DR EMBL; NWSH01002488; PCG68191.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A4J898; -.
DR STRING; 7102.A0A2A4J898; -.
DR Proteomes; UP000218220; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR CDD; cd17971; DEXHc_DHX8; 1.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044762; DHX8/Prp22_DEXHc.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000218220}.
FT DOMAIN 322..393
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 631..794
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 812..992
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 76..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 151..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 400..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..128
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..207
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..275
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 287..321
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 405..421
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1277 AA; 145205 MW; D809166B7031CBE2 CRC64;
MDEVAKLEHL SLVSKVCTEL DNHLGLNDKD LAEFIIDIAD KNPTFDSFKK ALIENGAEFS
DSFMTNLLRI IQHMKPSNTT QKAETSHEHS NPLANKFPGL AIPNEKPPKF SSDEESEEED
EKATKRLTAK EIFKDETKVK SSDAVVDQAM AELEALAPSQ GGPGKEPSKK DIPKEDIKKR
ERSHSRDRKD VSRRRERSRE RDRDRKSRRS RSRNKRSSSR DRRSRSRDRR SRSRGRRSRS
RNRDKDRRKR SRSRPTSSRH RSRDRHRRSK SRGKTSPSRG RTSPARGKRS PSREREHKYG
DYGKKSRKRS PEVEMSDDPE PGKIYNGRVA NIVPFGCFVQ IEGVRKRWEG LVHISQLRSE
GRVTNVSDVV NRGDKVKVKV LSVTGQKVSL TMKDVCQETG KDLNPASHAH LEAEREGRNP
DRPPPNATVL AGLQGGLDPD EDSSRKRVTR ISSPERWEIK QMISSGVIDK SELPDFDEET
GLLPKDEDGE ADIEIELVEE EPPFLQGHGR ALHDLSPVRI VKNPDGSLAQ AAMMQSALSK
ERREQKMIQR EQEMESLPMG LNKNWIDPLP ESDGRALAAN MRGTGITPQD LPEWKKHVIG
GKKSSFGKKT NLSLLEQRQS LPIYKLRDEL TKAVADNQIL IVIGETGSGK TTQITQYLAE
CGLTARGKIA CTQPRRVAAM SVAKRVAEEF GCRLGQEVGY TIRFEDCTSP ETVIKYMTDG
MLLRECLMDL DLKTYSVIML DEAHERTIHT DVLFGLLKQA VQKRPELKLI VTSATLDAVK
FSQYFFEAPI FTIPGRTFPV EVLYTKEPET DYLDASLITV MQIHLREPPG DILLFLTGQE
EIDTACEILY ERMKSLGPDV PELIILPVYS ALPSEMQTRI FEPAPPGSRK VVIATNIAET
SLTIDGIYYV VDPGFVKQKV YNSKTGMDSL VVTPISQAAA KQRAGRAGRT GPGKCYRLYT
ERAYRDEMLP TPVPEIQRTN LATTVLQLKT MGINDLLHFD FMDAPPVESL IMALEQLHSL
SALDSEGLLT RLGRRMAEFP LEPNLSKILI MSVALQCSDE ILTIVSMLSV QNVFYRPKDK
QALADQKKAK FNQPEGDHLT LLAVYNSWRN NKFSNAWCYE NFVQIRTLKR AQDVRKQLLG
IMDRHKLDVV SAGKNTVRIQ KTICSGFFRN AAKKDPQEGY RTLVDSQVVY IHPSSALFNR
QPEWVIYHEL VQTTKEYMRE VTTIDPKWLV EFAPAFFKFS DPTKLSKFKK NQRLEPLYNK
YEEPNAWRIS RVRRRRN
//
