ID A0A2A4KEE6_9ACTN Unreviewed; 491 AA.
AC A0A2A4KEE6;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
GN ORFNames=CIB93_31755 {ECO:0000313|EMBL:PCG82110.1};
OS Streptomyces sp. WZ.A104.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2023771 {ECO:0000313|EMBL:PCG82110.1, ECO:0000313|Proteomes:UP000217525};
RN [1] {ECO:0000313|EMBL:PCG82110.1, ECO:0000313|Proteomes:UP000217525}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WZ.A104 {ECO:0000313|EMBL:PCG82110.1,
RC ECO:0000313|Proteomes:UP000217525};
RA Xu L., He S., Yan X.;
RT "Isolation and Genomic Analysis of a Bioactive Actinomycete Associated with
RT Cyanobacterium Lyngbya cf. aestuarii.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000217525}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WZ.A104 {ECO:0000313|Proteomes:UP000217525};
RA Jiao Y., Han B.;
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC Evidence={ECO:0000256|ARBA:ARBA00034992};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC {ECO:0000256|ARBA:ARBA00009026}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PCG82110.1}.
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DR EMBL; NWVL01000085; PCG82110.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A4KEE6; -.
DR OrthoDB; 626362at2; -.
DR Proteomes; UP000217525; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.1610.20; Hen1, N-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR024026; 3'-RNA_MeTfrase_Hen1_bac.
DR InterPro; IPR026610; Hen1.
DR InterPro; IPR024740; Hen1_N.
DR InterPro; IPR038546; Hen1_N_sf.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR04074; bacter_Hen1; 1.
DR PANTHER; PTHR21404; HEN1; 1.
DR PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR Pfam; PF12623; Hen1_L; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:PCG82110.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000217525};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:PCG82110.1}.
FT DOMAIN 1..249
FT /note="Hen1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12623"
FT DOMAIN 303..405
FT /note="Methyltransferase type 12"
FT /evidence="ECO:0000259|Pfam:PF08242"
SQ SEQUENCE 491 AA; 54013 MW; 8A888DDAE283F336 CRC64;
MFLTIGTTGT PERPATDLGY LLHKHPGKAQ TFSTSHGPAH VFYPEASAER CTAALLLEVD
PVALVRRGKG KGRGGAPDAA LAQYVNDRPY AASSLLSVAL AAVFKSALGG VCRAMPERAE
QPLPLRIEVP ALPARGGVEL VHKLFAPLGW TRVEAVAVPL DEQFPEWGDS RYVRLVLEGE
LRLADALRQI YVLLPVLDDA KHYWVAPDEV DKLLRAGEGW LADHPERPLI TRRYLSHRWG
LTRQADQALE LVRLAESDDL EVESIDNAVD EGTDTEEKPV PLAEQRRTAI LDALRAAGAS
RVLDLGCGQG QLVQALLKDV RFTEIVGVDV SMRALTIASR RLKLDRMGER QADRVILRQG
SLTYTDKRLG GYDAAVLSEV VEHLDLPRLP ALEYAVFGAA RPGTVLVTTP NVEYNVRWET
LPAGHARHGD HRFEWTRAEF RDWAEQVAGR HGYTVRYVPV GPDDPEVGPP TQMAVFTRTT
ATEKTKEGKA A
//
