ID A0A2A4WVY2_9BACT Unreviewed; 582 AA.
AC A0A2A4WVY2;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 10-APR-2019, entry version 6.
DE RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993443};
DE EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00993444};
GN Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN ECO:0000313|EMBL:PCI73967.1};
GN ORFNames=COB28_03035 {ECO:0000313|EMBL:PCI73967.1};
OS Candidatus Dependentiae bacterium.
OC Bacteria; Candidatus Dependentiae.
OX NCBI_TaxID=2030827 {ECO:0000313|EMBL:PCI73967.1};
RN [1] {ECO:0000313|EMBL:PCI73967.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NORP98 {ECO:0000313|EMBL:PCI73967.1};
RX PubMed=29099490; DOI=10.1038/ismej.2017.187;
RA Tully B.J., Wheat C.G., Glazer B.T., Huber J.A.;
RT "A dynamic microbial community with high functional redundancy
RT inhabits the cold, oxic subseafloor aquifer.";
RL ISME J. 12:1-16(2018).
CC -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC molecules used as primers for DNA polymerase during DNA
CC replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC ECO:0000256|SAAS:SAAS00709340}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|SAAS:SAAS00709317};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC Rule:MF_00974};
CC -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
CC -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC domain that contains the primase activity, and a C-terminal DnaB-
CC binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC -!- SIMILARITY: Belongs to the DnaG primase family.
CC {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709351}.
CC -!- CAUTION: The sequence shown here is derived from an
CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC preliminary data. {ECO:0000313|EMBL:PCI73967.1}.
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DR EMBL; NVUD01000006; PCI73967.1; -; Genomic_DNA.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR Gene3D; 3.90.580.10; -; 1.
DR Gene3D; 3.90.980.10; -; 1.
DR HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR InterPro; IPR013264; DNA_primase_core_N.
DR InterPro; IPR037068; DNA_primase_core_N_sf.
DR InterPro; IPR006295; DNA_primase_DnaG.
DR InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR InterPro; IPR030846; DnaG_bac.
DR InterPro; IPR034151; TOPRIM_DnaG_bac.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR002694; Znf_CHC2.
DR Pfam; PF13662; Toprim_4; 1.
DR Pfam; PF08275; Toprim_N; 1.
DR Pfam; PF01807; zf-CHC2; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SMART; SM00400; ZnF_CHCC; 1.
DR TIGRFAMs; TIGR01391; dnaG; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993445};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709369};
KW DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709327};
KW Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709338};
KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709339};
KW Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709304};
KW Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709341};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00993442};
KW Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|SAAS:SAAS00709300};
KW Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW ECO:0000256|SAAS:SAAS00709301}.
FT DOMAIN 256 337 Toprim. {ECO:0000259|PROSITE:PS50880}.
FT ZN_FING 34 58 CHC2-type. {ECO:0000256|HAMAP-Rule:
FT MF_00974}.
FT COILED 540 560 {ECO:0000256|SAM:Coils}.
SQ SEQUENCE 582 AA; 66318 MW; 0D0EEA1C522EDC8C CRC64;
MTIFDHVKTS LSILEVVGSY VSLKRMGGYY KGPCPLHSEK DASFTVSPDR NIFYCFGCQV
GGDVISFIAR IERLTQGQAV RHLIEQHNLV IPESLQKEMS QHTETYVKES LSSVAKACAK
WAHQHFLNDE YAQKYLASRG MSEQMMKYFE IGLVAGGIKG MQRFIKGMQA ENILLDDLKS
VGFVMDGKTH PYSPFEDRIL FPIKDSIGRY IAFSGRVFLP EDDRARYYNS KESEMFSKRQ
TLFGFDMARK KAQQTNTIFL VEGGMDVVAM VQSGYENSVA TLGTACSADH LQMLRRFVHT
LVVVYDGDRA GQNAIEKVAE LCWDIAITLK VVSLPTGEDP ASLLQKGIPL NTFIEKQEDI
FSFLIKKTSK NFFNQSMPEK LRLCAQVVQM VKHATHGLKR HLLXQQISTA LQIPVAMLQK
VSXQELRKPT YELKEKEQQP TLSVQVAVLT GIEEKVTSFF FTADRQSSEY LKVYICMDEY
WNSPVQKIVE KWFQSPIADM HCFLDSLPES DRQLILSIVM KYGEGISYEV VETLMNELKK
RKWKAEVNRL KQDLDSARHE GDTARVHALI SNFLILKQKI QN
//
