ID A0A2A7MR51_MYCAG Unreviewed; 366 AA.
AC A0A2A7MR51;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Peptide chain release factor 1 {ECO:0000256|HAMAP-Rule:MF_00093};
DE Short=RF-1 {ECO:0000256|HAMAP-Rule:MF_00093};
GN Name=prfA {ECO:0000256|HAMAP-Rule:MF_00093,
GN ECO:0000313|EMBL:PEG33618.1};
GN ORFNames=CQY20_29775 {ECO:0000313|EMBL:PEG33618.1}, MAGR_28630
GN {ECO:0000313|EMBL:GFG51422.1};
OS Mycolicibacterium agri (Mycobacterium agri).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=36811 {ECO:0000313|EMBL:PEG33618.1, ECO:0000313|Proteomes:UP000220914};
RN [1] {ECO:0000313|EMBL:PEG33618.1, ECO:0000313|Proteomes:UP000220914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG37673 {ECO:0000313|EMBL:PEG33618.1,
RC ECO:0000313|Proteomes:UP000220914};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GFG51422.1, ECO:0000313|Proteomes:UP000465302}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 6377 {ECO:0000313|EMBL:GFG51422.1,
RC ECO:0000313|Proteomes:UP000465302};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:GFG51422.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 6377 {ECO:0000313|EMBL:GFG51422.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Peptide chain release factor 1 directs the termination of
CC translation in response to the peptide chain termination codons UAG and
CC UAA. {ECO:0000256|ARBA:ARBA00002986, ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- PTM: Methylated by PrmC. Methylation increases the termination
CC efficiency of RF1. {ECO:0000256|HAMAP-Rule:MF_00093}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000256|ARBA:ARBA00010835, ECO:0000256|HAMAP-
CC Rule:MF_00093}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PEG33618.1}.
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DR EMBL; BLKS01000001; GFG51422.1; -; Genomic_DNA.
DR EMBL; PDCP01000101; PEG33618.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A7MR51; -.
DR OrthoDB; 9806673at2; -.
DR Proteomes; UP000220914; Unassembled WGS sequence.
DR Proteomes; UP000465302; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.30.70.1660; -; 1.
DR Gene3D; 6.10.140.1950; -; 1.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR NCBIfam; TIGR00019; prfA; 1.
DR PANTHER; PTHR43804; LD18447P; 1.
DR PANTHER; PTHR43804:SF7; LD18447P; 1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; Release factor; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00093};
KW Methylation {ECO:0000256|ARBA:ARBA00022481, ECO:0000256|HAMAP-
KW Rule:MF_00093}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00093};
KW Reference proteome {ECO:0000313|Proteomes:UP000220914}.
FT DOMAIN 238..254
FT /note="Prokaryotic-type class I peptide chain release
FT factors"
FT /evidence="ECO:0000259|PROSITE:PS00745"
FT MOD_RES 245
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00093"
SQ SEQUENCE 366 AA; 40112 MW; A33D7CD07871E5B0 CRC64;
MTGITRAQSS AESGGGIEAI LSEHADLEKQ LSDPNLHSDA GRARKVGRRF AQLSPIVNTY
RKLEAARGDL EAARELAADD DSFADEVEEL TATVTELETQ LSDLLAPRDP HDPDDIVLEV
KSGEGGEESA LFAADLARMY IRYAERRGWT VTILDETTSD LGGYKDATLS IRSKGDSADG
VWSRLKFEGG VHRVQRVPVT ESQGRVHTSA AGVLVYPEPE EVEEVQIDES DLRIDVYRSS
GKGGQGVNTT DSAVRITHLP TGIVVTCQNE RSQLQNKIRA MQVLAARLQA MAEEQAQADA
SADRASQIRT VDRSERIRTY NFPENRIADH RINFKAHNLD QVLDGDLDPL LDALAAADRQ
ARLQQA
//
