ID A0A2A7MTV3_MYCAG Unreviewed; 1233 AA.
AC A0A2A7MTV3;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=CQY20_22470 {ECO:0000313|EMBL:PEG35242.1}, MAGR_47960
GN {ECO:0000313|EMBL:GFG53355.1};
OS Mycolicibacterium agri (Mycobacterium agri).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=36811 {ECO:0000313|EMBL:PEG35242.1, ECO:0000313|Proteomes:UP000220914};
RN [1] {ECO:0000313|EMBL:PEG35242.1, ECO:0000313|Proteomes:UP000220914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG37673 {ECO:0000313|EMBL:PEG35242.1,
RC ECO:0000313|Proteomes:UP000220914};
RA Tortoli E., Trovato A., Cirillo D.M.;
RT "The new phylogeny of genus Mycobacterium.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GFG53355.1, ECO:0000313|Proteomes:UP000465302}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 6377 {ECO:0000313|EMBL:GFG53355.1,
RC ECO:0000313|Proteomes:UP000465302};
RX PubMed=31287781;
RA Matsumoto Y., Kinjo T., Motooka D., Nabeya D., Jung N., Uechi K., Horii T.,
RA Iida T., Fujita J., Nakamura S.;
RT "Comprehensive subspecies identification of 175 nontuberculous mycobacteria
RT species based on 7547 genomic profiles.";
RL Emerg. Microbes Infect. 8:1043-1053(2019).
RN [3] {ECO:0000313|EMBL:GFG53355.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 6377 {ECO:0000313|EMBL:GFG53355.1};
RA Matsumoto Y., Motooka D., Nakamura S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PEG35242.1}.
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DR EMBL; BLKS01000001; GFG53355.1; -; Genomic_DNA.
DR EMBL; PDCP01000048; PEG35242.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A7MTV3; -.
DR OrthoDB; 9759518at2; -.
DR Proteomes; UP000220914; Unassembled WGS sequence.
DR Proteomes; UP000465302; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000220914}.
FT DOMAIN 53..117
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1233 AA; 136007 MW; 99BFD2BD483227E4 CRC64;
MTSPARIGGP IEELLERSGR FFTPGEFSAD LRTVTRRGGR DGDVFYRDRW SHDKVVRSTH
GVNCTGSCSW KIYVKDGIIT WETQETDYPS VGPDRPEYEP RGCPRGAAFS WYTYSPTRVR
YPYARGLLVE MYREAKARLG DPVLAWADIQ SGPERRRRYQ RARGKGGLVR VSWAEATEMI
AAAHVHTIKT YGPDRVAGFS PIPAMSMVSH AAGSRFVELI GGAMTSFYDW YADLPVASPQ
VFGDQTDVPE SGDWWDATYL MMWGSNVPVT RTPDAHWMVE VRYRGTKVVS VSPDYADNTK
FADEWMPCAA GADGALAMAM GHVILSECFV HQRVPFFVDY VRSYTDLPFL VKLEQRDGAL
VPGKNLTAAD LGHTTENAAF KPVLLDGKTD DVAVPHGSLG FRFGDDGVGK WNLDLGGLTP
ALTVARHEAG GETALIQLPR FDTADGHGEV LARGVPVRRV GEHLVCTVFD LMLAHYGVAR
PGLPGDWPTG YDDATHPYTP AWQEPITGVA AAQAERIAKE FARNAEESGG RSMIIMGAGI
CQWFHGDATY RAVLALLLLT GSMGRNGGGW AHYVGQEKCR PVTGWATMAM ATDWSRPPRQ
MAGTSYWYAH TDQWRYDGYR ADALASPLGR GRFRNKHTMD VLASAAAMGW SPFYPQFDRS
SLDVADEALA AGRDKSEIGA YVAEQLASGK LKLAVTDPDD PANWPRVLNI WRANLLGSSS
KGNEYFLRHL LGTDSNLQAK PTAEGLRPND VTWRDEIPEG KLDLLMSIDF RMTSTTLLSD
VVLPAATWYE KADLSSTDMH PYVHAFTPAI DPPWETHSDF EAFGAIARAF SALAKNHLGV
RTDIVVGALQ HDTPQAMAYP HGSENDWRTS AAVPVPGKTM GPVAVVERDY TAIADKWATL
GPLVEQLGVT TKAVTTHPER EVGELAAKFG VAKSGPAQGR PAITTDERMA DVILALSGTS
NGRLAVEGFR ELEKRTGRHL VHLAEGSEER RITYADAAAR PVPVITSPEW SGSETGGRRY
APFTVNIEEL KPFHTLTGRM HFYLDHDWLE ELGEQLPTYR PPLDMQRLFG EPQVGDARDG
IGLTVRYLTP HSKWSIHSEY QDNLLMLSLS RGGPTMWMSP ADAAKIDVRD NDWVEAVNRN
GVLVCRAIVS HRMPEGVVFV YHVQERTIDV PLSETTGLRG GIHNTLTRLL IKPSHLAGGY
AQNAFAFNYL GPTGNQRDEV TVVRRRSQEV TYQ
//
