ID   A0A2A8HNR4_9SPHN        Unreviewed;       395 AA.
AC   A0A2A8HNR4;
DT   20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT   20-DEC-2017, sequence version 1.
DT   27-MAR-2024, entry version 14.
DE   SubName: Full=Glutathione-dependent formaldehyde dehydrogenase {ECO:0000313|EMBL:PEQ10572.1};
GN   ORFNames=B2G71_21555 {ECO:0000313|EMBL:PEQ10572.1};
OS   Novosphingobium sp. PC22D.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Novosphingobium.
OX   NCBI_TaxID=1962403 {ECO:0000313|EMBL:PEQ10572.1, ECO:0000313|Proteomes:UP000220675};
RN   [1] {ECO:0000313|EMBL:PEQ10572.1, ECO:0000313|Proteomes:UP000220675}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PC22D {ECO:0000313|EMBL:PEQ10572.1,
RC   ECO:0000313|Proteomes:UP000220675};
RA   Peterson S.W.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947,
CC         ECO:0000256|RuleBase:RU361277};
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. {ECO:0000256|RuleBase:RU361277}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PEQ10572.1}.
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DR   EMBL; MWMO01000022; PEQ10572.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2A8HNR4; -.
DR   OrthoDB; 9773078at2; -.
DR   Proteomes; UP000220675; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd08283; FDH_like_1; 1.
DR   Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR013154; ADH-like_N.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR42813:SF2; DEHYDROGENASE, ZINC-CONTAINING, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02810)-RELATED; 1.
DR   PANTHER; PTHR42813; ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SUPFAM; SSF50129; GroES-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|RuleBase:RU361277};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000220675};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU361277}.
FT   DOMAIN          26..150
FT                   /note="Alcohol dehydrogenase-like N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF08240"
FT   DOMAIN          197..265
FT                   /note="Alcohol dehydrogenase-like C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00107"
SQ   SEQUENCE   395 AA;  42543 MW;  3C915CA3895B4350 CRC64;
     MRALAYHGKH DVRVDTVDDP EIVNPRDAII KVTSTAICGS DLHLYDGVIP AVLEGDILGH
     EFMGEVVETG PKSTLEKGQR VVVPFTISCG QCFHCQIQQY SCCDNSNPAE KQDMSATLYG
     HSMAGLFGYS HLTGGYSGGQ AEYVRVPYSD IGPIAVPDHL DDDQVLFLSD ILPTGWMAAE
     NADIGPDDTI AVWGCGPVGL FAVQSALAMG AAEVIAIDHY PHRLELARKL GAKTINFRES
     NVREALMEMS GGIGVDAVID AVGMESHGFA IDNMLDIAKQ TIGVGADRAS VLKQAILAVR
     RGGRVSIPGV YGGMTDKFPL GALMEKGLQV RGGQTHVQKY TKDLLAKIED GTLDTTFLIS
     HRLPLEDAAK GYKNFKEQQD VWTKVVLKPG METQA
//