ID A0A2A9D176_9MICO Unreviewed; 1235 AA.
AC A0A2A9D176;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ATL40_2058 {ECO:0000313|EMBL:PFG20458.1};
OS Serinibacter salmoneus.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Beutenbergiaceae;
OC Serinibacter.
OX NCBI_TaxID=556530 {ECO:0000313|EMBL:PFG20458.1, ECO:0000313|Proteomes:UP000224915};
RN [1] {ECO:0000313|EMBL:PFG20458.1, ECO:0000313|Proteomes:UP000224915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 21801 {ECO:0000313|EMBL:PFG20458.1,
RC ECO:0000313|Proteomes:UP000224915};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PFG20458.1}.
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DR EMBL; PDJD01000001; PFG20458.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A9D176; -.
DR Proteomes; UP000224915; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000224915}.
FT DOMAIN 692..853
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 878..1028
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1235 AA; 134150 MW; C1C5311851B60EB3 CRC64;
MGFGRPSGTP RLGGREAATL GPAAQNTPMK LTGLLSALAH DPSIDPTLRA LAQRRACEVI
APVGARPALI AQLALAPQAP QIVVLTATGR EAESLVAALG AYLPAESLAA LPAWETLPHE
RLSPRADTVA TRLAVMRRLT HPDPEGTGGS HTGRIRVLVV PVRAAMQPVV TGLGDLEPVA
AHAGQTRPME EIVEALAAAA YTRTDMVTAR GEFAVRGGIL DVFPPTQDHP MRLDFWGDEI
EEIRWFSAAD QRSLEVAPEG LWAPPCRELL LTDEVRERAL RLRDALPGAA EMLEKMAQGI
AVEGMESLTP ALVDEMSQVL DLVEDDALLM AWDPERIRSR AADLQATTEE FLAAAWTSAA
AGGSVPLDLS AASFVALAQV YERAQARGLL RWSLTVLPSD ADLVGADDTA GDEENLRLAA
RDVEGYRGDL ERAVSDLRDL VRQEWRLVLA TDAAGQARRM VEQLRAAQVP ARLESDLETA
PEPGVVHVTT ATIGRGFVAP DLRLALFTEA DLTGRATAGA RDAKRLASRR RNVVDPLALR
PGDYVVHEQH GVGRFVELVQ RTVGGAAGAR GKPATREYLV IEYASPKQGK NAGSQDLLYV
PTDALDQVTK YTGGEAPTLS RMGGSDWKNT KARARKAVRE IAGELIRLYS ARMATQGHAF
APDTPWQREL EDAFAYVETP DQLVTIEEVK ADMEKSVPMD RLICGDVGYG KTEVAVRAAF
KAVQDGKQVA VLVPTTLLVQ QHLQTFTDRF TGFPVTVAAL SRFQTDAEAA RVREDVRAGK
VDVVIGTHRL LTGEVRFKDL GLVIVDEEQR FGVEHKETLK QLRTNVDVLA MSATPIPRTL
EMAVTGIREM STLATPPEER HPVLTFVGPY EEKQIAAAIR RELLREGQVF YVHNRVESIE
RAAAHLAELV PEARIAVAHG KMNEHQLERV IMDFWEKRFD VLVCTTIVET GLDISNANTL
ILERADVMGL SQLHQLRGRV GRGRERAYSY FLYPPEKPLT ETAHDRLATI AANTDLGAGM
QVALKDLEIR GAGNLLGGEQ SGHIAGVGFD LYVRMVSEAV QRFRGEEVEE LQDVTIELPI
DAHIPHEYVP HERLRLEAYQ KIASARGSED VAAVREELVD RYGAVPASTE LLFAVAEMRT
LARSLGVTAI TIQGQYIRFA PVELSDSATV RLKRLYPRSV IKAAVRTILV PAPTTARVGG
EVLRDLALVE WVMGLLRSVV GSSVAAAAAV AGPGS
//
