ID A0A2A9E0F9_9MICO Unreviewed; 799 AA.
AC A0A2A9E0F9;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=ATJ78_3055 {ECO:0000313|EMBL:PFG32071.1};
OS Microbacterium agarici.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=630514 {ECO:0000313|EMBL:PFG32071.1, ECO:0000313|Proteomes:UP000221369};
RN [1] {ECO:0000313|EMBL:PFG32071.1, ECO:0000313|Proteomes:UP000221369}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 21798 {ECO:0000313|EMBL:PFG32071.1,
RC ECO:0000313|Proteomes:UP000221369};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PFG32071.1}.
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DR EMBL; PDJE01000001; PFG32071.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2A9E0F9; -.
DR Proteomes; UP000221369; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000221369}.
FT DOMAIN 45..338
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 339..612
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 66..73
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 799 AA; 87876 MW; 24B6D982712C484F CRC64;
MTSTSTSDAH GSEQVTDDPA TPVIVDWPTA GIPGDGSAAS AHLLDGLNPQ QREAVEYRGK
ALLIVAGAGS GKTRVLTHRI ASLIENREAW PSQILAITFT NKAAAEMRER VEDLLGGRTE
GMWISTFHSA CVRILRREAE NVGFGKNFTI YDSSDSRVLL KRIIKRYDAD VLGFTPANAA
AKISKLKNEL ADADSYARNA NMSDPNEAAF VEIFRDYQRE LQRASAFDFD DLIAQTVYLF
RAFPQVAALY QKKFRHVLVD EYQDTNHAQY ALIRELTREP EADGFLPGVD EGASLTVVGD
SDQSIYAFRG ADIRNIVEFE RDFRGAKVIL LEQNYRSTQN ILSAANAVIA NNFDRKDKKL
WTDIGSGDKI IGFTGYSGHD EAQFVADEIE RLHADGLAYG DIAVFYRTNA QTRALEEIFV
RSALPYKIMG GTKFYERAEI KDAFAYLTSV MNPADELAVR RIINTPKRGI GPATVTQLAS
FAENAETTLR EALREASTLG FGPKVTRAIS DLSNVLDEAA ALADAGKVSE VLTTLLQKSG
LIDALRMSRD PQDEARAENL DELVAVTKEF ERNNPDGTLI DFLTEVSLVA AADDIDDASG
TVSLMTLHTA KGLEYDTVFL TGVEEDLLPH RMSANEPGGP SEERRLFYVG ITRARKRLFL
SLAMTRAQFG ETAVAMPSRY LQEIPAELID WKQSPGSVNS RGGTQSRALN ARRWSPGRGQ
DAAALPRAEK KQWPNKVTNT VRDNGDLELA AGDRIRHSDF GEGRVQNVTG EGTKRVAHVL
FDAAGAKKLL IKIAPIEKL
//
