ID A0A2B4RWL1_STYPI Unreviewed; 680 AA.
AC A0A2B4RWL1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE AltName: Full=ATP-binding domain-containing protein 4 {ECO:0000256|ARBA:ARBA00031202};
DE AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
DE AltName: Full=Protein DPH6 homolog {ECO:0000256|ARBA:ARBA00032849};
GN Name=dph6 {ECO:0000313|EMBL:PFX20718.1};
GN ORFNames=AWC38_SpisGene14821 {ECO:0000313|EMBL:PFX20718.1};
OS Stylophora pistillata (Smooth cauliflower coral).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC Astrocoeniina; Pocilloporidae; Stylophora.
OX NCBI_TaxID=50429 {ECO:0000313|EMBL:PFX20718.1, ECO:0000313|Proteomes:UP000225706};
RN [1] {ECO:0000313|Proteomes:UP000225706}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Voolstra C.R., Li Y., Liew Y.J., Baumgarten S., Zoccola D., Flot J.-F.,
RA Tambutte S., Allemand D., Aranda M.;
RT "Comparative analysis of the genomes of Stylophora pistillata and Acropora
RT digitifera provides evidence for extensive differences between species of
RT corals.";
RL bioRxiv 0:0-0(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC ChEBI:CHEBI:456215; EC=6.3.1.14;
CC Evidence={ECO:0000256|ARBA:ARBA00001559};
CC -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005156}.
CC -!- SIMILARITY: Belongs to the Diphthine--ammonia ligase family.
CC {ECO:0000256|ARBA:ARBA00008496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PFX20718.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LSMT01000306; PFX20718.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2B4RWL1; -.
DR STRING; 50429.A0A2B4RWL1; -.
DR EnsemblMetazoa; XM_022942639.1; XP_022798374.1; LOC111336530.
DR UniPathway; UPA00559; -.
DR Proteomes; UP000225706; Unassembled WGS sequence.
DR GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0017183; P:protein histidyl modification to diphthamide; IEA:UniProtKB-UniPathway.
DR CDD; cd01994; Alpha_ANH_like_IV; 1.
DR CDD; cd06155; eu_AANH_C_1; 1.
DR CDD; cd06156; eu_AANH_C_2; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR Gene3D; 3.30.1330.40; RutC-like; 2.
DR InterPro; IPR002761; Diphthami_syn_dom.
DR InterPro; IPR030662; DPH6/MJ0570.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR035959; RutC-like_sf.
DR InterPro; IPR006175; YjgF/YER057c/UK114.
DR NCBIfam; TIGR00290; MJ0570_dom; 1.
DR PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR Pfam; PF01902; Diphthami_syn_2; 1.
DR Pfam; PF01042; Ribonuc_L-PSP; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF55298; YjgF-like; 2.
PE 3: Inferred from homology;
KW Ligase {ECO:0000313|EMBL:PFX20718.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000225706}.
FT DOMAIN 2..232
FT /note="Diphthamide synthase"
FT /evidence="ECO:0000259|Pfam:PF01902"
SQ SEQUENCE 680 AA; 75670 MW; 3655412046034EAF CRC64;
MMRVVALISG GKDSCYNMMQ CVQQGHTIIA LANLKPRGDA DELDSYMYQT VGHNAIDLYA
QAMDLPLYRG TIEGSSVDQG KVYTESQGDE VEDLYKLLKN IKEKIEFDAV STGAILSDYQ
RVRVEHVCSR LGLSSLAYLW RRDQTELLGE MISSGVEAIL IKVAAMDLSP KRHLGLPIAD
VYSLMCNLKD KYGSNVCGEG GEYETFTLDC PLFKKRIVIE ESESVIHSDD AFAPVGFLCF
KKIHVEDKDI DKVNSELGLK NHKCEELFDK LQILPDNTTD IKEKGIAERP LNIKIPKIQP
ELGKELRGRI LYSRCGDYIW LSGIYGSCDN EGKKLAIEDV TRKVFQSLKD TLTSLKASLS
DGVMVHLFVK NMDDFAKVNS VYKSFLSVNP PARACIQLNL PEDVSIQVDC LVYAAEGASN
SMREAMHVQS ISHWAPANIG PYSQATKFDI SHMGDAMAGK MVRDTIFISG SIGLWPPTMS
MVTGGISMEA PLSLQHVDRI LTALNVAKSL QNVVHGFCFL TSSVFVPVAK NAWSHALKDK
EGLLLVQDNS TLADRGSFSV HVQSIAVKGN INSSFAVNIA VESTSTNFTL EEVMVTFISE
IKCYTSQHYF SLSHLMYLRI FYVKGIMDHW NAQDFIHRSL ERNTLLVPTF SLIPVDALEK
NTVMLFCCFL QKAENESLVD
//
