ID A0A2B4SCP1_STYPI Unreviewed; 1171 AA.
AC A0A2B4SCP1;
DT 20-DEC-2017, integrated into UniProtKB/TrEMBL.
DT 20-DEC-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Long-chain-fatty-acid--CoA ligase {ECO:0000256|ARBA:ARBA00041297};
GN Name=SLC27A4 {ECO:0000313|EMBL:PFX26215.1};
GN ORFNames=AWC38_SpisGene9113 {ECO:0000313|EMBL:PFX26215.1};
OS Stylophora pistillata (Smooth cauliflower coral).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC Astrocoeniina; Pocilloporidae; Stylophora.
OX NCBI_TaxID=50429 {ECO:0000313|EMBL:PFX26215.1, ECO:0000313|Proteomes:UP000225706};
RN [1] {ECO:0000313|Proteomes:UP000225706}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Voolstra C.R., Li Y., Liew Y.J., Baumgarten S., Zoccola D., Flot J.-F.,
RA Tambutte S., Allemand D., Aranda M.;
RT "Comparative analysis of the genomes of Stylophora pistillata and Acropora
RT digitifera provides evidence for extensive differences between species of
RT corals.";
RL bioRxiv 0:0-0(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + CoA + tetracosanoate = AMP + diphosphate +
CC tetracosanoyl-CoA; Xref=Rhea:RHEA:33639, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:31014, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:65052, ChEBI:CHEBI:456215;
CC Evidence={ECO:0000256|ARBA:ARBA00036436};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33640;
CC Evidence={ECO:0000256|ARBA:ARBA00036436};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very long-chain fatty acid + ATP + CoA = a very long-chain
CC fatty acyl-CoA + AMP + diphosphate; Xref=Rhea:RHEA:54536,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58950, ChEBI:CHEBI:138261, ChEBI:CHEBI:456215;
CC Evidence={ECO:0000256|ARBA:ARBA00036527};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54537;
CC Evidence={ECO:0000256|ARBA:ARBA00036527};
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000256|ARBA:ARBA00006432}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PFX26215.1}.
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DR EMBL; LSMT01000131; PFX26215.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2B4SCP1; -.
DR STRING; 50429.A0A2B4SCP1; -.
DR EnsemblMetazoa; XM_022933897.1; XP_022789632.1; LOC111329262.
DR Proteomes; UP000225706; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1410.40; -; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.30.460.90; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR024810; Mab-21-like.
DR PANTHER; PTHR43107:SF15; FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A; 1.
DR PANTHER; PTHR43107; LONG-CHAIN FATTY ACID TRANSPORT PROTEIN; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SMART; SM01265; Mab-21; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000225706};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 26..43
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 106..471
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 543..618
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
SQ SEQUENCE 1171 AA; 132622 MW; 21454E76C6F76A89 CRC64;
MQGRTDCLKK LSFPTRVVRH IKSRKMYYVW AAIVASFATI VVYFRLPFIV FPVLLFVAFL
ASGGKDFPRV FFRTILRDIK GFSRFANLLI TTRLHVKNKH TISDIFQETV SKHPQKAAIV
FEKRTWTFKE VDEYSNRIAN FFKSQGYQKG DVVALYLENC PEFICIWLGL SKLGVVTALI
NTNLRSDSLF HCISVASVRA VIFGTDLSGP MKEIRTKIPE VVELYSLGVK STFMASVVHL
NQKIEASSPV MSAVNHPKCF ADVLLYIYTS GTTGLPKAAV VTTARYFRNA FGVICALKVR
NDDVVYCPMP LYHSAAGIVG VGMCFIAGCT LSLRRKFSAS NFWDDCVETK ATVIQYIGEL
CRYLLSQPHR PSENKHSVRL AIGNGLRPQI WKEFQERFSI KEIGEFYGST EGTASVINID
STPGACGFMS EILPSVYPVV IFKVDPDTGE LVRGPDGLAV RTKPGEVGQI VGKSLRGDPS
LRFDGYLNSL ETKKKIAHDV FRKGDAAFLS GDLVFRDEFG YIYFHDRTGD TFRWRGENVS
TAEVESVMSK VLGLRDVVVY GVLVPGTEGR AGMAAIVDPE YKVDLAELAA SLKKLLPSYA
CPMFLRIVHS VDLTGTFKLQ KSKLRNEGFD IGVIEDQLFY YDSKEKTYLA LDEVKYEQII
RGEIRIAETM DHMHRAAQQI VKQVVDNIGR IKPHLDVKEV ISMGSFAEGT KICSPNEFDF
MACFDFLSRN ENVRIEGTEG CQPGYMVAFL NSGFDKAMAN ITRLLYKDIC CIHHEGLRAE
FVETLKEVVK AMLMKKVVTP HGIMVIKGSD YLALKLEWHK FKENYEGPEI YNGLCDRDLI
LATSYSLDIG VDIMPAVSVE DLTPLSDIHG FPSHVFGMTG NVKFHLVCKV SGQSPHAPYL
HISHATTEVF LVRHLHPIHK KCYRVLKYLL THNTYFNRPI KDISLSSYVF KTAVLFHEYE
KLCSGTPDIV TCCIEIIRYV RVKLREGVFP PFLMRTTNVW GQCYKIPVSD AWRPSGLDRE
ICSFDWCFVL WFQLWRQFLG KALTIFQKIK LKLQTEEPET SDSSTLLNDA SLTSLNSMSQ
TRDLVEYVPN RYDSFLAEFE VLREDAFVIT TKYSKDAKDT QTFSFISRCP SEKVWELVLP
KFPEYLSRIE SVCSREMSIP KEEFEEVVEV G
//
